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Literature summary extracted from

  • Pluskal, T.; Ueno, M.; Yanagida, M.
    Genetic and metabolomic dissection of the ergothioneine and selenoneine biosynthetic pathway in the fission yeast, S. pombe, and construction of an overproduction system (2014), PLoS ONE, 9, e97774 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.10 gene egt1+, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree Schizosaccharomyces pombe
4.4.1.36 gene egt2+, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.10 additional information construction of an egt1+ overexpression system by replacing its native promoter with the nmt1+ promoter, which is inducible in the absence of thiamine. Generation of a egt1+ deletion mutant, DELTAegt1, by replacing the target loci in the wild-type 972 strain with the kanamycin resistance marker (kanMX) leading to absence of all ergothioneine pathway intermediates and ergothioneine itself in DELTAegt1. Employment of three versions of the nmt1 promoter plasmid with increasing strength of expression and constructed three strains P81nmt1-egt1+, P41nmt1-egt1+, and P3nmt1-egt1+, respectively. Mutant DELTAegt1 strain shows no growth defects during cultivation in either rich (YE) or minimal (EMM2) culture media, deletion of gene egt1+ causes no significant perturbation to the intracellular metabolome of quiescent cells. No sensitivity or resistance of the mutant strains to oxidative stress compared to wild-type 972 strain Schizosaccharomyces pombe
4.4.1.36 additional information construction of an egt2+ overexpression system by replacing its native promoter with the nmt1+ promoter, which is inducible in the absence of thiamine. Generation of a egt2+ deletion mutant, DELTAegt2, by replacing the target loci in the wild-type 972 strain with the kanamycin resistance marker (kanMX). Mutant DELTASPBC660.12c, designated DELTAegt2, shows a substantial decrease in ergothioneine, accompanied by accumulation of its immediate precursor, hercynylcysteine sulfoxide. Ergothioneine-deficient strains exhibit no phenotypic defects during vegetative growth or quiescence. Construction of multiple deletion mutants of SPBC660.12c (egt2+) with the other candidate EgtE homologues, but even in successfully constructed double and triple mutants, a significant amount of EGT still remains, because hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'phosphate. Mutant DELTAegt1 strain shows no growth defects during cultivation in either rich (YE) or minimal (EMM2) culture media, deletion of gene egt1+ causes no significant perturbation to the intracellular metabolome of quiescent cells Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.10 Fe2+ required for catalysis Schizosaccharomyces pombe
4.4.1.36 Fe2+ required for catalysis Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.10 hercynine + L-cysteine + O2 Schizosaccharomyces pombe
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 hercynine + L-cysteine + O2 Schizosaccharomyces pombe 972
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 additional information Schizosaccharomyces pombe the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 ?
-
 ?
1.21.3.10 additional information Schizosaccharomyces pombe 972 the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 ?
-
 ?
4.4.1.36 additional information Schizosaccharomyces pombe the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead ?
-
 ?
4.4.1.36 additional information Schizosaccharomyces pombe 972 the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead ?
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Schizosaccharomyces pombe overall reaction ergothioneine + pyruvate + ammonia + acceptor
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Schizosaccharomyces pombe 972 overall reaction ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.50 no activity in Neurospora crassa
-
-
-
1.21.3.10 Schizosaccharomyces pombe O94632
-
-
1.21.3.10 Schizosaccharomyces pombe 972 O94632
-
-
4.4.1.36 Schizosaccharomyces pombe O94431
-
-
4.4.1.36 Schizosaccharomyces pombe 972 O94431
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.10 hercynine + L-cysteine + O2
-
 Schizosaccharomyces pombe S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 hercynine + L-cysteine + O2
-
 Schizosaccharomyces pombe 972 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 Schizosaccharomyces pombe ?
-
 ?
1.21.3.10 additional information hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'-phosphate Schizosaccharomyces pombe ?
-
 ?
1.21.3.10 additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 Schizosaccharomyces pombe 972 ?
-
 ?
1.21.3.10 additional information hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'-phosphate Schizosaccharomyces pombe 972 ?
-
 ?
4.4.1.36 additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead Schizosaccharomyces pombe ?
-
 ?
4.4.1.36 additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead Schizosaccharomyces pombe 972 ?
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Schizosaccharomyces pombe ergothioneine + pyruvate + ammonia + acceptor
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Schizosaccharomyces pombe 972 ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.10 More Egt-1 domain structure, overview Schizosaccharomyces pombe
4.4.1.36 More Egt-2 domain structure, overview Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
1.21.3.10 Egt-1
-
 Schizosaccharomyces pombe
1.21.3.10 egt1+
-
 Schizosaccharomyces pombe
1.21.3.10 ergothioneine biosynthesis protein 1
-
 Schizosaccharomyces pombe
1.21.3.10 mug158+/SPBC1604.01 locus name Schizosaccharomyces pombe
1.21.3.10 protein SPBC1604.01
-
 Schizosaccharomyces pombe
1.21.3.10 SPBC1604.01 locus name Schizosaccharomyces pombe
4.4.1.36 Egt-2
-
 Schizosaccharomyces pombe
4.4.1.36 Egt2
-
 Schizosaccharomyces pombe
4.4.1.36 egt2+
-
 Schizosaccharomyces pombe
4.4.1.36 egtE
-
 Schizosaccharomyces pombe
4.4.1.36 ergothioneine biosynthesis protein 2
-
 Schizosaccharomyces pombe
4.4.1.36 hercynylcysteine sulfoxide lyase UniProt Schizosaccharomyces pombe
4.4.1.36 SPBC660.12c locus name Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
1.21.3.10 evolution mug158+/SPBC1604.01 is a distant homologue of the mycobacterial EgtD and EgtB genes, encoding a single fusion protein. The Schizosaccharomyces pombe homologue utilizes cysteine as a substrate, rather than using gamma-glutamyl-cysteine, as in the case of the bacterial EgtB enzyme, EC 1.14.99.50 Schizosaccharomyces pombe
1.21.3.10 metabolism the enzyme produces hercynylcysteine sulfoxide in the ergothioneine pathway. The ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis involves an intermediate compound, hercynylselenocysteine Schizosaccharomyces pombe
1.21.3.10 physiological function contribution of gene egt1+ to oxidative stress response, but gene egt1+ might not be among the primary mechanisms that protect Schizosaccharomyces pombe from exogenous peroxide Schizosaccharomyces pombe
4.4.1.36 metabolism SPBC660.12c locus egt2+ represents the gene primarily responsible for the second step of ergothioneine biosynthesis in Schizosaccharomyces pombe Schizosaccharomyces pombe
4.4.1.36 physiological function a gene deletion mutant shows a substantial decrease in ergothioneine, accompanied by accumulation of its immediate precursor, hercynylcysteine sulfoxide. Ergothioneine-deficient strains exhibit no phenotypic defects during vegetative growth or quiescence. The ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium Schizosaccharomyces pombe