Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, K.R.; An, J.U.; Lee, S.H.; Oh, D.K.
    Selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by a lipoxygenase from Nostoc sp. SAG 25.82 (2015), PLoS ONE, 10, e0137785 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.61 acetone activates best at 5% v/v Nostoc sp.
1.13.11.61 additional information no effect by Brij58 Nostoc sp.
1.13.11.61 Tween 20
-
 Nostoc sp.
1.13.11.61 Tween 40
-
 Nostoc sp.
1.13.11.61 Tween 80 activates best at 0.2% w/v Nostoc sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.61 gene 9R-lox, recombinant expression of His-tagged enzyme in Escherichia coli Nostoc sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.61 additional information no effect by Brij58; no effects by Brij58 Nostoc sp.
1.13.11.61 SDS
-
 Nostoc sp.
1.13.11.61 SPAN 20 slight inhibition Nostoc sp.
1.13.11.61 Span 40 slight inhibition Nostoc sp.
1.13.11.61 Span 60 slight inhibition Nostoc sp.
1.13.11.61 SPAN 80 slight inhibition Nostoc sp.
1.13.11.61 Triton X-100
-
 Nostoc sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.61 additional information
-
 additional information Michaelis-Menten kinetics Nostoc sp.
1.13.11.61 0.054
-
 alpha-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 0.054
-
 gamma-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 0.404
-
 linoleic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.61 156000
-
 recombinant His-tagged enzyme, gel filtration Nostoc sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.61 alpha-linolenic acid + O2 Nostoc sp.
-
 (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid
-
 ?
1.13.11.61 alpha-linolenic acid + O2 Nostoc sp. SAG 25.82
-
 (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid
-
 ?
1.13.11.61 gamma-linolenic acid + O2 Nostoc sp.
-
 (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid
-
 ?
1.13.11.61 gamma-linolenic acid + O2 Nostoc sp. SAG 25.82
-
 (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid
-
 ?
1.13.11.61 linoleic acid + O2 Nostoc sp.
-
 (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid
-
 ?
1.13.11.61 linoleic acid + O2 Nostoc sp. SAG 25.82
-
 (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.61 Nostoc sp.
-
-
-
1.13.11.61 Nostoc sp. SAG 25.82
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.61 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Nostoc sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.61 5.4
-
 recombinant enzyme, pH 8.5, 15°C, substrate gamma-linolenic acid Nostoc sp.
1.13.11.61 6.3
-
 recombinant enzyme, pH 8.5, 15°C, substrate alpha-linolenic acid Nostoc sp.
1.13.11.61 18.4
-
 recombinant enzyme, pH 8.5, 15°C, substrate linolenic acid Nostoc sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.61 alpha-linolenic acid + O2
-
 Nostoc sp. (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid
-
 ?
1.13.11.61 alpha-linolenic acid + O2
-
 Nostoc sp. SAG 25.82 (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid
-
 ?
1.13.11.61 alpha-linolenic acid + O2 optimal reaction conditions are pH 8.5, 15°C, 5% v/v acetone, 0.2% w/v Tween 80, 40 g/l alpha-linolenic acid, and 1 g/l enzyme, assay method optimization Nostoc sp. (9R,10E,12Z,15Z)-9-hydroxy-octadeca-10,12,15-trienoic acid
-
 ?
1.13.11.61 alpha-linolenic acid + O2 optimal reaction conditions are pH 8.5, 15°C, 5% v/v acetone, 0.2% w/v Tween 80, 40 g/l alpha-linolenic acid, and 1 g/l enzyme, assay method optimization Nostoc sp. SAG 25.82 (9R,10E,12Z,15Z)-9-hydroxy-octadeca-10,12,15-trienoic acid
-
 ?
1.13.11.61 gamma-linolenic acid + O2
-
 Nostoc sp. (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid
-
 ?
1.13.11.61 gamma-linolenic acid + O2
-
 Nostoc sp. SAG 25.82 (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid
-
 ?
1.13.11.61 linoleic acid + O2
-
 Nostoc sp. (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid
-
 ?
1.13.11.61 linoleic acid + O2
-
 Nostoc sp. SAG 25.82 (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid
-
 ?
1.13.11.61 additional information selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by the enzyme from Nostoc sp. strain SAG 25.82, heterologously expressed in Escherichia coli. The specific activity of the enzyme followed the descending order linoleic acid > alpha-linolenic acid > gamma-linolenic acid. Product determination by LC-MS/MS analysis Nostoc sp. ?
-
 ?
1.13.11.61 additional information selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by the enzyme from Nostoc sp. strain SAG 25.82, heterologously expressed in Escherichia coli. The specific activity of the enzyme followed the descending order linoleic acid > alpha-linolenic acid > gamma-linolenic acid. Product determination by LC-MS/MS analysis Nostoc sp. SAG 25.82 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.61 homotrimer 3 * 52000, recombinant His-tagged enzyme, SDS-PAGE, 3 * 52300, about, sequence calculation Nostoc sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.61 9R-lipoxygenase
-
 Nostoc sp.
1.13.11.61 9R-LOX
-
 Nostoc sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.61 15
-
-
 Nostoc sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.61 0.097
-
 gamma-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 0.13
-
 alpha-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 5.12
-
 linoleic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.61 8.5
-
-
 Nostoc sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.61 4.5 10.5 50% of maximal activity at pH 4.5-6.5, maximal activity at pH 8.5 Nostoc sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.61 1.82
-
 gamma-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 2.4
-
 alpha-linolenic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.
1.13.11.61 12.67
-
 linoleic acid recombinant enzyme, pH 8.5, 15°C Nostoc sp.