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Literature summary extracted from

  • Schmidt, D.; Rizzi, V.; Gaziola, S.A.; Medici, L.O.; Vincze, E.; Kozak, M.; Lea, P.J.; Azevedo, R.A.
    Lysine metabolism in antisense C-hordein barley grains (2015), Plant Physiol. Biochem., 87, 73-83 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.8 additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines, overview Hordeum vulgare
1.5.1.9 additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines., overview Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines., overview Hordeum vulgare
1.5.1.9 additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines., overview Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH + H+ Hordeum vulgare
-
 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Hordeum vulgare
-
 L-glutamate + (S)-2-amino-6-oxohexanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Hordeum vulgare
-
 cv. Golden Promise
-
1.5.1.9 Hordeum vulgare
-
 cv. Golden Promise
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 native enzyme partially through ammonium sulfate fractionation and desalting gel filtration Hordeum vulgare
1.5.1.9 native enzyme partially through ammonium sulfate fractionation and desalting gel filtration Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 seed grain, spikes, at different developmental stages: early milk, late milk, and soft dough. The enzyme exhibits the highest activity at the late milk stage Hordeum vulgare
-
1.5.1.9 seed grain, spikes, at different developmental stages: early milk, late milk, and soft dough. The enzyme exhibits the highest activity at the late milk stage Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 0.00039
-
 enzyme from grain at late milk stage, pH 7.4, 30°C Hordeum vulgare
1.5.1.9 0.00227
-
 enzyme from grain at late milk stage, pH 8.4, 30°C Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH + H+
-
 Hordeum vulgare N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Hordeum vulgare L-glutamate + (S)-2-amino-6-oxohexanoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.8 LKR
-
 Hordeum vulgare
1.5.1.8 lysine alpha-ketoglutarate reductase
-
 Hordeum vulgare
1.5.1.9 saccharopine dehydrogenase
-
 Hordeum vulgare
1.5.1.9 SDH
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.8 30
-
 assay at Hordeum vulgare
1.5.1.9 30
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.8 7.4
-
 assay at Hordeum vulgare
1.5.1.9 8.4
-
 assay at Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADPH
-
 Hordeum vulgare
1.5.1.9 NAD+
-
 Hordeum vulgare

Expression

EC Number Organism Comment Expression
1.5.1.8 Hordeum vulgare C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines, overview additional information
1.5.1.9 Hordeum vulgare C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines, overview additional information

General Information

EC Number General Information Comment Organism
1.5.1.8 metabolism the enzyme is involved in L-lysine degradation Hordeum vulgare
1.5.1.9 metabolism the enzyme is involved in L-lysine degradation Hordeum vulgare