Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, G.Y.; Kim, H.M.; Ma, S.H.; Park, S.H.; Joung, Y.H.; Yun, C.H.
    Heterologous expression and functional characterization of the NADPH-cytochrome P450 reductase from Capsicum annuum (2014), Plant Physiol. Biochem., 82, 116-122 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 isoform CPR1 is expressed in Escherichia coli Rosetta(DE3)pLysS cells Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.081
-
 ferricytochrome c isoform CPR1, at pH 7.6 and 25°C Capsicum annuum

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Capsicum annuum A0A0N6XHR4 cultivar Bukang
-
1.6.2.4 Capsicum annuum B5LAU2 cultivar Bukang
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 2',5'-ADP Sepharose column chromatography Capsicum annuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 flower
-
 Capsicum annuum
-
1.6.2.4 fruit
-
 Capsicum annuum
-
1.6.2.4 leaf
-
 Capsicum annuum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 NADPH + H+ + doxorubicin
-
 Capsicum annuum NADP+ + 7-deoxydoxorubicinone
-
 ?
1.6.2.4 NADPH + H+ + ferricyanide
-
 Capsicum annuum NADP+ + ferrocyanide
-
 ?
1.6.2.4 NADPH + H+ + ferricytochrome b5
-
 Capsicum annuum NADP+ + ferrocytochrome b5
-
 ?
1.6.2.4 NADPH + H+ + ferricytochrome c
-
 Capsicum annuum NADP+ + ferrocytochrome c
-
 ?
1.6.2.4 NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
 Capsicum annuum NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
 ?
1.6.2.4 NADPH + H+ + oxidized 5-cyano-2,3-ditolyl tetrazolium chloride
-
 Capsicum annuum NADP+ + reduced 5-cyano-2,3-ditolyl tetrazolium chloride
-
 ?
1.6.2.4 NADPH + H+ + oxidized tetrazolium
-
 Capsicum annuum NADP+ + reduced tetrazolium
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 76000, SDS-PAGE Capsicum annuum

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR1 isoform Capsicum annuum
1.6.2.4 CPR2 isoform Capsicum annuum
1.6.2.4 NADPH-cytochrome P450 reductase
-
 Capsicum annuum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 56
-
 melting temperature Capsicum annuum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 15.7
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide isoform CPR1, at pH 7.6 and 25°C Capsicum annuum
1.6.2.4 45.7
-
 ferricytochrome c isoform CPR1, at pH 7.6 and 25°C Capsicum annuum
1.6.2.4 80
-
 ferricyanide isoform CPR1, at pH 7.6 and 25°C Capsicum annuum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Capsicum annuum
1.6.2.4 FMN
-
 Capsicum annuum

Expression

EC Number Organism Comment Expression
1.6.2.4 Capsicum annuum at the red ripe stage, the expression level of isoform CPR1 is more than 6fold greater than that in leaves or flowers up