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Literature summary extracted from

  • Liu, F.; Guo, X.; Yao, Y.; Tang, W.; Zhang, W.; Cao, S.; Han, Y.; Liu, Y.
    Cloning and function characterization of two dehydroascorbate reductases from kiwifruit (Actinidia chinensis L.) (2016), Plant Mol. Biol. Rep., 34, 815-826 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells, Arabidopsis thaliana, and Nicotiana benthamiana Actinidia chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.07
-
 dehydroascorbate isoform DHAR2, at pH 7.8 and 25°C Actinidia chinensis
1.8.5.1 0.11
-
 dehydroascorbate isoform DHAR1, at pH 7.8 and 25°C Actinidia chinensis
1.8.5.1 1.27
-
 glutathione isoform DHAR1, at pH 7.8 and 25°C Actinidia chinensis
1.8.5.1 2.22
-
 glutathione isoform DHAR2, at pH 7.8 and 25°C Actinidia chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 chloroplast isoform DHAR1 Actinidia chinensis 9507
-
1.8.5.1 cytosol isoform DHAR1 Actinidia chinensis 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate Actinidia chinensis
-
 glutathione disulfide + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Actinidia chinensis
-
 cultivar Hongyang
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 Ni-NTA column chromatography Actinidia chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.1 leaf
-
 Actinidia chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate
-
 Actinidia chinensis glutathione disulfide + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.1 ? x * 28000, isoform DHAR1, SDS-PAGE Actinidia chinensis
1.8.5.1 ? x * 32000, isoform DHAR2, SDS-PAGE Actinidia chinensis

Synonyms

EC Number Synonyms Comment Organism
1.8.5.1 dehydroascorbate reductase
-
 Actinidia chinensis
1.8.5.1 DHAR1 isoform Actinidia chinensis
1.8.5.1 DHAR2 isoform Actinidia chinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 35 55 when preincubated at 35°C for 15 min, the enzyme activity is almost unaffected but begins to decrease when the temperature sets above 40°C. Isoform DHAR1 and DHAR2 still retain 61 and 73% of their initial activity when pre-incubated at 55°C for 15 min Actinidia chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7
-
-
 Actinidia chinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.5.1 6 10 isoforms DHAR1 and DHAR2 retain more than 80% of their maximum enzymatic activities at pH values between 6.0 and 10.0, respectively Actinidia chinensis