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Literature summary extracted from

  • Tripathy, J.N.; Hirasawa, M.; Sutton, R.B.; Dasgupta, A.; Vaidyanathan, N.; Zabet-Moghaddam, M.; Florencio, F.J.; Srivastava, A.P.; Knaff, D.B.
    A loop unique to ferredoxin-dependent glutamate synthases is not absolutely essential for ferredoxin-dependent catalytic activity (2015), Photosynth. Res., 123, 129-139 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.7.1 expressed in Escherichia coli Synechocystis sp. PCC 6803

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.7.1 0.0006
-
 reduced ferredoxin wild type enzyme, at pH 8.0, temperature not specified in the publication Synechocystis sp. PCC 6803
1.4.7.1 0.04
-
 2-oxoglutarate wild type enzyme, at pH 8.0, temperature not specified in the publication Synechocystis sp. PCC 6803
1.4.7.1 1.3
-
 L-glutamine wild type enzyme, at pH 8.0, temperature not specified in the publication Synechocystis sp. PCC 6803

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.7.1 Iron 3 mol iron per mol enzyme Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+ Synechocystis sp. PCC 6803
-
 L-glutamate + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.7.1 Synechocystis sp. PCC 6803 P55038
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.7.1 Ni-NTA column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+
-
 Synechocystis sp. PCC 6803 L-glutamate + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.7.1 ferredoxin-dependent glutamate synthase
-
 Synechocystis sp. PCC 6803
1.4.7.1 glutamine:oxoglutarate amidotransferase
-
 Synechocystis sp. PCC 6803
1.4.7.1 GOGAT
-
 Synechocystis sp. PCC 6803

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.7.1 Ferredoxin
-
 Synechocystis sp. PCC 6803
1.4.7.1 FMN
-
 Synechocystis sp. PCC 6803