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Literature summary extracted from

  • Karnaouri, A.; Topakas, E.; Paschos, T.; Taouki, I.; Christakopoulos, P.
    Cloning, expression and characterization of an ethanol tolerant GH3 beta-glucosidase from Myceliophthora thermophila (2013), PeerJ, 1, e46 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Co2+ 10 mM, 85% residual activity Thermothelomyces thermophilus
3.2.1.21 EDTA 10 mM, 90% residual activity Thermothelomyces thermophilus
3.2.1.21 SDS 10 mM, 91% residual activity Thermothelomyces thermophilus
3.2.1.21 Urea 10 mM, 95% residual activity Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.39
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Thermothelomyces thermophilus
3.2.1.21 2.64
-
 cellobiose pH 5.0, 50°C Thermothelomyces thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Thermothelomyces thermophilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Cu2+ 10 mM, 208% of initial activity Thermothelomyces thermophilus
3.2.1.21 Mn2+ 10 mM, 167% of initial activity Thermothelomyces thermophilus
3.2.1.21 Zn2+ 10 mM, 149% of initial activity Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermothelomyces thermophilus G2QDN2
-
-
3.2.1.21 Thermothelomyces thermophilus ATCC 42464 G2QDN2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 20.6
-
 substrate lichenan. It , pH 5.0, 50°C Thermothelomyces thermophilus
3.2.1.21 30.7
-
 substrate cellobiose, pH 5.0, 50°C Thermothelomyces thermophilus
3.2.1.21 52
-
 substrate laminarin, pH 5.0, 50°C Thermothelomyces thermophilus
3.2.1.21 97.7
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 50°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus ATCC 42464 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermothelomyces thermophilus 2 D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermothelomyces thermophilus ATCC 42464 2 D-glucose
-
 ?
3.2.1.21 laminarin + H2O
-
 Thermothelomyces thermophilus laminarioligosaccharides + D-glucose
-
 ?
3.2.1.21 laminarin + H2O
-
 Thermothelomyces thermophilus ATCC 42464 laminarioligosaccharides + D-glucose
-
 ?
3.2.1.21 lichenan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.21 lichenan + H2O
-
 Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
3.2.1.21 additional information enzyme hydrolyzes low molecular weight substrates and polysaccharides containing beta-glucosidic residues, and efficiently synthesizes methyl-D-glucoside in the presence of methanol due to its transglycosylation activity. It shows no detectable activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, carboxymethyl cellulose, wheat and birchwood xylans and avicel Thermothelomyces thermophilus ?
-
 ?
3.2.1.21 additional information enzyme hydrolyzes low molecular weight substrates and polysaccharides containing beta-glucosidic residues, and efficiently synthesizes methyl-D-glucoside in the presence of methanol due to its transglycosylation activity. It shows no detectable activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, carboxymethyl cellulose, wheat and birchwood xylans and avicel Thermothelomyces thermophilus ATCC 42464 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 90000, SDS-PAGE, x * 79819, calculated Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 bgl3a
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 70
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 half-life 143 min Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 6
-
 more than 80% of maximum activity Thermothelomyces thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 3 7 24 h, almost 90% residual activity Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Thermothelomyces thermophilus calculated
-
 5.1