EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.221 | expression in Escherichia coli | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.221 | 0.0508 | - |
ATP | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 0.08 | - |
N-acetyl-D-muramate | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 0.177 | - |
N-acetyl-D-glucosamine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.221 | ATP + N-acetyl-D-galactosamine | Pseudomonas putida | - |
ADP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-galactosamine | Pseudomonas putida DSM 6125 | - |
ADP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-glucosamine | Pseudomonas putida | - |
ADP + N-acetyl-D-glucosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-glucosamine | Pseudomonas putida DSM 6125 | - |
ADP + N-acetyl-D-glucosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-muramate | Pseudomonas putida | - |
ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-muramate | Pseudomonas putida DSM 6125 | - |
ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.1.221 | additional information | Pseudomonas putida | no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine | ? | - |
? | |
2.7.1.221 | additional information | Pseudomonas putida DSM 6125 | no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.221 | Pseudomonas putida | Q88QT3 | member of protein family PF01636 | - |
2.7.1.221 | Pseudomonas putida DSM 6125 | Q88QT3 | member of protein family PF01636 | - |
2.7.7.99 | Pseudomonas putida | Q88QT2 | - |
- |
2.7.7.99 | Pseudomonas putida DSM 6125 | Q88QT2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.221 | ATP + N-acetyl-D-galactosamine | - |
Pseudomonas putida | ADP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-galactosamine | - |
Pseudomonas putida DSM 6125 | ADP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-glucosamine | - |
Pseudomonas putida | ADP + N-acetyl-D-glucosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-glucosamine | - |
Pseudomonas putida DSM 6125 | ADP + N-acetyl-D-glucosamine 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-muramate | - |
Pseudomonas putida | ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.1.221 | ATP + N-acetyl-D-muramate | - |
Pseudomonas putida DSM 6125 | ADP + N-acetyl-alpha-D-muramate 1-phosphate | - |
? | |
2.7.1.221 | additional information | no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine | Pseudomonas putida | ? | - |
? | |
2.7.1.221 | additional information | no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine | Pseudomonas putida DSM 6125 | ? | - |
? | |
2.7.7.99 | additional information | no substrates: N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-galactosamine 1-phosphate | Pseudomonas putida | ? | - |
? | |
2.7.7.99 | additional information | no substrates: N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-galactosamine 1-phosphate | Pseudomonas putida DSM 6125 | ? | - |
? | |
2.7.7.99 | UTP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida | UDP-N-acetyl-alpha-D-muramate + diphosphate | - |
? | |
2.7.7.99 | UTP + N-acetyl-alpha-D-muramate 1-phosphate | - |
Pseudomonas putida DSM 6125 | UDP-N-acetyl-alpha-D-muramate + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.221 | amgK | - |
Pseudomonas putida |
2.7.1.221 | pp_0405 | - |
Pseudomonas putida |
2.7.7.99 | murU | - |
Pseudomonas putida |
2.7.7.99 | N-acetylmuramate alpha-1-phosphate uridylyltransferase | - |
Pseudomonas putida |
2.7.7.99 | pp_0406 | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.221 | 4.38 | - |
ATP | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 5.15 | - |
N-acetyl-D-muramate | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida | |
2.7.1.221 | 6.64 | - |
N-acetyl-D-glucosamine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.221 | physiological function | enzyme is involved in a cell wall sugar-recycling route that channels external MurNAc directly to peptidoglycan biosynthesis. A deletion mutant shows increase fosfomycin sensitivity | Pseudomonas putida |
2.7.7.99 | physiological function | enzyme is involved in a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid. The pathway consisits of anomeric sugar kinase AmgK and the MurNAc alpha-1-phosphate uridylyl transferase MurU. Deletion of the encoding gene increases fosfomycin sensitivity | Pseudomonas putida |