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Literature summary extracted from

  • Ito, H.; Otsuki, A.; Mori, H.; Li, P.; Kinoshita, M.; Kawakami, Y.; Tsuji, H.; Fang, D.; Takahashi, Y.
    Two new monoterpene glycosides from Qing Shan Lu Shui tea with inhibitory effects on leukocyte-type 12-lipoxygenase activity (2013), Molecules, 18, 4257-4266 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 recombinant overexpression in J774A.1 cells Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octa-dienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-cis-p-coumaroyl)-beta-D-glucopyranoside liguroside B, NMR spectral analysis Sus scrofa
1.13.11.31 (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octadienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-trans-p-coumaroyl)-beta-D-glucopyranoside liguroside A, NMR spectral analysis Sus scrofa
1.13.11.31 epigallocatechin gallate
-
 Sus scrofa
1.13.11.31 kudingoside B NMR spectral analysis Sus scrofa
1.13.11.31 additional information Chinese tea Qing Shan Lu Shui, which contains lower catechin levels than the other tested teas, inhibits the enzyme activity, 12-LOX inhibitory activity–guided fractionation of the aqueous ethanol extract are used to isolate twp components: monoterpene glycosides liguroside A and liguroside B, i.e. (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octadienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-trans-p-coumaroyl)-beta-D-glucopyranoside and (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octa-dienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-cis-p-coumaroyl)-beta-D-glucopyranoside, respectively. Inhibitory effect on leukocyte-type 12-LOX activity and catechin contents of 12 Chinese teas, overview Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.31 arachidonate + O2 Sus scrofa
-
 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Sus scrofa P16469
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 recombinant enzyme partiallay from J774A.1 cells Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 leukocyte
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
 Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 leukocyte-type 12-lipoxygenase
-
 Sus scrofa
1.13.11.31 leukocyte-type 12-LOX
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.31 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.4
-
 assay at Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.31 0.0007
-
 pH 7.4, 30°C, recombinant leukocyte-type enzyme Sus scrofa (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octa-dienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-cis-p-coumaroyl)-beta-D-glucopyranoside
1.13.11.31 0.0017
-
 pH 7.4, 30°C, recombinant leukocyte-type enzyme Sus scrofa (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octadienyl-O-(alpha-L-rhamnopyranosyl)-(1''->3')-(4'''-O-trans-p-coumaroyl)-beta-D-glucopyranoside