Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Subbotina, N.; Kolomytseva, M.; Baskunov, B.; Golovlev, L.
    Catechol 1,2-dioxygenase induced in Rhodococcus opacus strain 1CP cultured in the presence of 3-hydroxybenzoate (2016), Microbiology (Russian Federation), 85, 638-641 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.00185
-
 catechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 0.0035
-
 4-methylcatechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 0.0048
-
 3-methylcatechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 0.0093
-
 1,2,3-benzenetriol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 0.64
-
 4-Chlorocatechol at pH 7.2 and 45°C Rhodococcus opacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Rhodococcus opacus
-
 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 Rhodococcus opacus 1CP
-
 cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Rhodococcus opacus
-
-
-
1.13.11.1 Rhodococcus opacus 1CP
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 DEAE-Toyopearl column chromatography, phenyl Sepharose CL-4B column chromatography, and Resource Q column chromatography Rhodococcus opacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 37.65
-
 after 319fold purification, at pH 7.2 and 45°C Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-chlorocatechol + O2
-
 Rhodococcus opacus ?
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Rhodococcus opacus ?
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Rhodococcus opacus 1CP ?
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Rhodococcus opacus ?
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Rhodococcus opacus 1CP ?
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Rhodococcus opacus ?
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Rhodococcus opacus 1CP ?
-
 ?
1.13.11.1 catechol + O2
-
 Rhodococcus opacus cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Rhodococcus opacus 1CP cis,cis-muconate
-
 ?
1.13.11.1 pyrogallol + O2
-
 Rhodococcus opacus ?
-
 ?
1.13.11.1 pyrogallol + O2
-
 Rhodococcus opacus 1CP ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 ? x * 32000, SDS-PAGE Rhodococcus opacus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C1,2-DO
-
 Rhodococcus opacus
1.13.11.1 catechol:oxygen 1,2-oxidoreductase
-
 Rhodococcus opacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 45
-
-
 Rhodococcus opacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5 8
-
 Rhodococcus opacus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.1 0.133
-
 4-Chlorocatechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 458
-
 1,2,3-benzenetriol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 3305
-
 3-methylcatechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 5547
-
 4-methylcatechol at pH 7.2 and 45°C Rhodococcus opacus
1.13.11.1 10845
-
 catechol at pH 7.2 and 45°C Rhodococcus opacus