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Literature summary extracted from

  • Veetil, V.P.; Angermayr, S.A.; Hellingwerf, K.J.
    Ethylene production with engineered Synechocystis sp PCC 6803 strains (2017), Microb. Cell Fact., 16, 34 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.19 gene efe, recombinant enzyme expression in Synechocystis sp. strain PCC 6803, stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3) as the ribosome-binding site, at the slr0168 neutral site. Increase in ethylene production is achieved twofold by RBS screening, improvement of ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-hostself-replicating plasmid, which has a higher copy number than the genome Pseudomonas savastanoi pv. phaseolicola

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.19 additional information construction of a glycogen-synthesis knockout mutant (DELTAglgC) to raise the intracellular amounts of the key Efe substrate, 2-oxoglutarate, from which ethylene is formed. Introduction of the ethylene biosynthetic pathway in mutant DELTAglgC. Under nitrogen limiting conditions, the glycogen knockout strain has increased intracellular 2-oxoglutarate levels, but the ethylene production is lower in the mutant strain than in the wild-type strain Pseudomonas savastanoi pv. phaseolicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2 Pseudomonas savastanoi pv. phaseolicola
-
 ethylene + 3 CO2 + H2O
-
 ?
1.13.12.19 2-oxoglutarate + O2 Pseudomonas savastanoi pv. phaseolicola PK2
-
 ethylene + 3 CO2 + H2O
-
 ?
1.14.20.7 2-oxoglutarate + L-arginine + O2 Synechocystis sp. PCC 6803
-
 succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.19 Pseudomonas savastanoi pv. phaseolicola Q549K5 the Kudzu strain
-
1.13.12.19 Pseudomonas savastanoi pv. phaseolicola PK2 Q549K5 the Kudzu strain
-
1.14.20.7 Synechocystis sp. PCC 6803
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2
-
 Pseudomonas savastanoi pv. phaseolicola ethylene + 3 CO2 + H2O
-
 ?
1.13.12.19 2-oxoglutarate + O2
-
 Pseudomonas savastanoi pv. phaseolicola PK2 ethylene + 3 CO2 + H2O
-
 ?
1.14.20.7 2-oxoglutarate + L-arginine + O2
-
 Synechocystis sp. PCC 6803 succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.19 ethylene-forming enzyme
-
 Pseudomonas savastanoi pv. phaseolicola
1.14.20.7 EFE
-
 Synechocystis sp. PCC 6803
1.14.20.7 ethylene forming enzyme
-
 Synechocystis sp. PCC 6803

General Information

EC Number General Information Comment Organism
1.13.12.19 physiological function the ethylene-forming enzyme (Efe) from Pseudomonas syringae pv. phaseolicola PK2 (the Kudzu strain) catalyzes the conversion of the ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene Pseudomonas savastanoi pv. phaseolicola