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Literature summary extracted from

  • Ong, K.; Liew, S.; Mutalib, S.; Murad, A.; Bakar, F.
    Isolation and cloning of an aryl-aldehyde dehydrogenase gene from the white-rot fungus Pycnoporus cinnabarinus strain MUCL 39533 (2015), Malays. J. Microbiol., 11, 391-397 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.2.1.29 synthesis the enzyme can be used for production of bio-vanillin from vanillic acid Trametes cinnabarina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.29 gene PcALDH, cloning from RNA via RT-PCR, sequence comparisons and phylogenetic analysis, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 Trametes cinnabarina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.29 additional information enzyme PcALDH contains a signal peptide Trametes cinnabarina
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.29 vanillin + NAD+ Trametes cinnabarina
-
 vanillinic acid + NADH + H+
-
 r
1.2.1.29 vanillin + NAD+ Trametes cinnabarina MUCL 39533
-
 vanillinic acid + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.29 Trametes cinnabarina A0A0U2ETT9
-
-
1.2.1.29 Trametes cinnabarina MUCL 39533 A0A0U2ETT9
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.29 an aromatic aldehyde + NAD+ + H2O = an aromatic acid + NADH + H+ ALDH catalysis involves acylation and deacylation Trametes cinnabarina
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.29 mycelium
-
 Trametes cinnabarina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.29 additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina ?
-
 ?
1.2.1.29 additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina MUCL 39533 ?
-
 ?
1.2.1.29 vanillin + NAD+
-
 Trametes cinnabarina vanillinic acid + NADH + H+
-
 r
1.2.1.29 vanillin + NAD+
-
 Trametes cinnabarina MUCL 39533 vanillinic acid + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.29 PcALDH
-
 Trametes cinnabarina

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.29 NAD+
-
 Trametes cinnabarina
1.2.1.29 NADH
-
 Trametes cinnabarina

General Information

EC Number General Information Comment Organism
1.2.1.29 evolution in silico analysis of PcALDH indicate that enzyme PcALDH belongs to the ALDH superfamily and class 3 ALDHs Trametes cinnabarina
1.2.1.29 metabolism in filamentous fungi, vanillin is formed in a two-stage process in which ferulic acid is converted to vanillic acid then reduced to vanillin Trametes cinnabarina
1.2.1.29 physiological function reduction of vanillic acid to vanillin is catalysed by the key enzyme aryl-aldehyde dehydrogenase Trametes cinnabarina