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Literature summary extracted from

  • Wang, Y.; Li, J.; Wu, Q.; Zhu, D.
    Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp. and Rhodococcus erythropolis (2013), J. Mol. Catal. B, 88, 14-19 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.B51 synthesis the enzyme can be used for synthesis of optically pure 3-quinuclidinol. Optically pure 3-quinuclidinol is an important intermediate for the synthesis of various anticholinergic drugs. (R)-3-Quinuclidinol is used to synthesize muscarinic M1 or M3 receptor antagonists such as talsaclidine, revatropate, and solifenacin Nocardia sp. WY1202
1.1.1.B63 synthesis the enzyme can be used for synthesis of optically pure 3-quinuclidinol. Optically pure 3-quinuclidinol is an important intermediate for the synthesis of various anticholinergic drugs. (S)-3-Quinuclidinol is a very promising chiral building blocks for synthesis of serotonin receptor antagonist drugs and anticholinergic drugs Rhodococcus erythropolis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B51 phylogenetic tree Nocardia sp. WY1202
1.1.1.B63 phylogenetic tree Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B51 3-quinuclidinone + NADPH + H+ Nocardia sp. WY1202 stereospecific reduction of 3-quinuclidinone (R)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ Rhodococcus erythropolis stereospecific reduction of 3-quinuclidinone (S)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ Rhodococcus erythropolis WY1406 stereospecific reduction of 3-quinuclidinone (S)-3-quinuclidinol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B51 Nocardia sp. WY1202
-
-
-
1.1.1.B63 Rhodococcus erythropolis
-
-
-
1.1.1.B63 Rhodococcus erythropolis WY1406
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.B51 additional information apart from D-glucose and glycerol, various sugars and alcohols like maltose, sucrose, arabinose, mannitol, sorbitol are not utilized by strain WY1202 for growth Nocardia sp. WY1202
-
1.1.1.B63 additional information strain WY1406 utilizes most of the tested sugars, i.e. D-glucose, glycerol, maltose, sucrose, arabinose, sorbitol, except mannitol, for growth Rhodococcus erythropolis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B51 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone Nocardia sp. WY1202 (R)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B51 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone with over 85-95% enantiomeric excess, effects of concentration of 3-quinuclidinone hydrochloride, overview Nocardia sp. WY1202 (R)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone Rhodococcus erythropolis (S)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone with over 99% enantiomeric excess, effects of concentration of 3-quinuclidinone hydrochloride, overview Rhodococcus erythropolis (S)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone Rhodococcus erythropolis WY1406 (S)-3-quinuclidinol + NADP+
-
 ?
1.1.1.B63 3-quinuclidinone + NADPH + H+ stereospecific reduction of 3-quinuclidinone with over 99% enantiomeric excess, effects of concentration of 3-quinuclidinone hydrochloride, overview Rhodococcus erythropolis WY1406 (S)-3-quinuclidinol + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B51 30
-
-
 Nocardia sp. WY1202
1.1.1.B63 37
-
-
 Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B51 8
-
-
 Nocardia sp. WY1202
1.1.1.B63 7 8
-
 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B51 NADPH
-
 Nocardia sp. WY1202
1.1.1.B63 NADPH
-
 Rhodococcus erythropolis