Any feedback?
Literature summary extracted from
- Purification and characterization of an alkaline chloride-tolerant laccase from a halotolerant bacterium, Bacillus sp. strain WT (2016), J. Mol. Catal. B, 134, 89-97 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.2 | Ba2+ | 1 mM, 11% loss of activity | Bacillus sp. (in: Bacteria) |  |
| 1.10.3.2 | EDTA | 1 mM, 15.6% loss of activity | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | L-cysteine | 1 mM, reduced 87% of its original activity | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | additional information | the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | SDS | 1 mM, 38.6% loss of activity | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 0.0037 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | 0.1327 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.10.3.2 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.2 | Na+ | up to 400 mM NaCl stimulates laccase activity. At 600 to 800 mM NaCl, the laccase retains all of its original activity and at higher concentrations, the activity decreases | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | NaCl | halotolerant with maximum activity at 100 mM NaCl | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | Ni2+ | 1 mM, 12% increase in activity | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 180000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.2 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 1.10.3.2 | Bacillus sp. (in: Bacteria) WT | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.2 | - |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 1 | - |
substrate: syringaldazine, pH 8.0, 22°C | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | 2,6-dimethoxyphenol + O2 | 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | alpha-naphthol + O2 | 22.0% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
| 1.10.3.2 | guaiacol + O2 | 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | L-DOPA + O2 | 13.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
| 1.10.3.2 | pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
| 1.10.3.2 | tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | ? | x * 180000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 55 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or syringaldazine | Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 50 | 80 | 50°C: about 20% of maximal activity, 90°C: about 40% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 50 | - |
a significant increase in activity is observed until 60 min, in which the relative activity reaches to 150% | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 60 | - |
after 15 min incubation relative activity reaches to 140% | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 70 | - |
after 15 min incubation relative activity reaches to 148% | Bacillus sp. (in: Bacteria) |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 51 | - |
syringaldazine | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | 309 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 8 | - |
substrate: syringaldazine | Bacillus sp. (in: Bacteria) |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 4 | 6 | pH 4.0: about 70% of maximal activity, pH 6.0: about 85% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 2328 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | 13783 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
