EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B65 | DTT | enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM | Pseudomonas putida | |
1.1.1.B65 | Triton X-100 | 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B65 | Ca2+ | inhibits 4% aT 1 mM and 33% at 10 mM | Pseudomonas putida | |
1.1.1.B65 | EDTA | inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively | Pseudomonas putida | |
1.1.1.B65 | Hg2+ | strong inhibition at 1 mM | Pseudomonas putida | |
1.1.1.B65 | Li+ | inhibits 17% at 10 mM | Pseudomonas putida | |
1.1.1.B65 | Mg2+ | inhibits 19% at 1 mM and 31% at 10 mM | Pseudomonas putida | |
1.1.1.B65 | Mn2+ | 21% activation at 1 mM, and 61% inhibition at 10 mM | Pseudomonas putida | |
1.1.1.B65 | Ni2+ | inhibits 39% at 1 mM and 63% at 10 mM | Pseudomonas putida | |
1.1.1.B65 | SDS | strongly inhibits the enzyme activity at 0.25-0.75% | Pseudomonas putida | |
1.1.1.B65 | Triton X-100 | 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity | Pseudomonas putida | |
1.1.1.B65 | Zn2+ | strong inhibition at 10 mM | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.B65 | 0.015 | - |
NADP+ | pH 8.5, 30°C | Pseudomonas putida | |
1.1.1.B65 | 0.018 | - |
NAD+ | pH 8.5, 30°C | Pseudomonas putida | |
1.1.1.B65 | 0.02 | - |
(R)-mandelate | pH 8.5, 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B65 | K+ | activates 44% at 1 mM and 69% at 10 mM | Pseudomonas putida | |
1.1.1.B65 | Mn2+ | 21% activation at 1 mM, and 61% inhibition at 10 mM | Pseudomonas putida | |
1.1.1.B65 | Na+ | activates 12% at 10 mM | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B65 | (R)-mandelate + NAD+ | Pseudomonas putida | - |
phenylglyoxylate + NADH + H+ | - |
r | |
1.1.1.B65 | (R)-mandelate + NAD+ | Pseudomonas putida NUST506 | - |
phenylglyoxylate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.B65 | Pseudomonas putida | - |
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China) | - |
1.1.1.B65 | Pseudomonas putida NUST506 | - |
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China) | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.B65 | native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 0.03 | - |
crude enzyme extract, pH 8.5, 30°C | Pseudomonas putida |
1.1.1.B65 | 0.33 | - |
purified native enzyme, pH 8.5, 30°C | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.B65 | 4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B65 | (R)-mandelate + NAD+ | - |
Pseudomonas putida | phenylglyoxylate + NADH + H+ | - |
r | |
1.1.1.B65 | (R)-mandelate + NAD+ | - |
Pseudomonas putida NUST506 | phenylglyoxylate + NADH + H+ | - |
r | |
1.1.1.B65 | (R)-mandelate + NADP+ | - |
Pseudomonas putida | phenylglyoxylate + NADPH + H+ | - |
r | |
1.1.1.B65 | (R)-mandelate + NADP+ | - |
Pseudomonas putida NUST506 | phenylglyoxylate + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.B65 | ? | x * 61000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.B65 | RMDH | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 30 | - |
- |
Pseudomonas putida |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 20 | 55 | activity range, profile overview | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 30 | - |
purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.B65 | 0.3 | - |
NADP+ | pH 8.5, 30°C | Pseudomonas putida | |
1.1.1.B65 | 0.9 | - |
NAD+ | pH 8.5, 30°C | Pseudomonas putida | |
1.1.1.B65 | 0.9 | - |
(R)-mandelate | pH 8.5, 30°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 8.5 | - |
- |
Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 5 | 10 | activity range, profile overview | Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B65 | 6 | 9 | purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B65 | NAD+ | - |
Pseudomonas putida | |
1.1.1.B65 | NADH | - |
Pseudomonas putida | |
1.1.1.B65 | NADP+ | - |
Pseudomonas putida | |
1.1.1.B65 | NADPH | - |
Pseudomonas putida |