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Literature summary extracted from

  • Schüttmann, I.; Bouws, H.; Szweda, R.; Suckow, M.; Czermak, P.; Zorn, H.
    Induction, characterization, and heterologous expression of a carotenoid degrading versatile peroxidase from Pleurotus sapidus (2014), J. Mol. Catal. B, 103, 79-84 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expressed in Hansenula polymorpha strain RB11 Pleurotus sapidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.05
-
 beta-carotene at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 0.14
-
 syringol at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 0.17
-
 veratryl alcohol at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 0.18
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at 30°C and pH 4.5 Pleurotus sapidus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus sapidus Q4QZ27
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.16 DEAE FF' column chromatography and Superdex 200 gel filtration Pleurotus sapidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.16 0.4
-
 with beta-carotene as substrate, at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 1.2
-
 with syringol as substrate, at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 1.4
-
 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as substrate, at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 2.3
-
 with veratryl alcohol as substrate, at 30°C and pH 4.5 Pleurotus sapidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H+ + H2O2
-
 Pleurotus sapidus ?
-
 ?
1.11.1.16 beta-carotene + H+ + H2O2
-
 Pleurotus sapidus ?
-
 ?
1.11.1.16 additional information no activity with Phenol Red and Remazol Brilliant Blue Pleurotus sapidus ?
-
 ?
1.11.1.16 syringol + H+ + H2O2
-
 Pleurotus sapidus ?
-
 ?
1.11.1.16 veratryl alcohol + H+ + H2O2
-
 Pleurotus sapidus veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.16 ? x * 38000, SDS-PAGE Pleurotus sapidus
1.11.1.16 ? x * 34600, calculated from amino acid sequence Pleurotus sapidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.16 30
-
-
 Pleurotus sapidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 1000
-
 beta-carotene at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 1600
-
 veratryl alcohol at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 12300
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at 30°C and pH 4.5 Pleurotus sapidus
1.11.1.16 54500
-
 syringol at 30°C and pH 4.5 Pleurotus sapidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.16 4.5
-
-
 Pleurotus sapidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.16 3 6.5 more than 60% activity between pH 3.0 and 6.5 Pleurotus sapidus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.16 Pleurotus sapidus isoelectric focusing
-
 3.6