Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ai, M.Q.; Wang, F.F.; Huang, F.
    Purification and characterization of a thermostable laccase from Trametes trogii and its ability in modification of Kraft lignin (2015), J. Microbiol. Biotechnol., 25, 1361-1370 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 industry potential use of the laccase in lignin modification Coriolopsis trogii
1.10.3.2 synthesis potential use of the laccase in lignin modification Coriolopsis trogii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 AlCl3 10 mM, 40% inhibition Coriolopsis trogii
1.10.3.2 CaCl2 10 mM, 31% inhibition Coriolopsis trogii
1.10.3.2 CoSO4 10 mM, 9% inhibition Coriolopsis trogii
1.10.3.2 CuSO4 10 mM, 19% inhibition Coriolopsis trogii
1.10.3.2 dithiothreitol 0.1 mM, complete inhibition Coriolopsis trogii
1.10.3.2 EDTA 10 mM, 6% inhibition Coriolopsis trogii
1.10.3.2 FeCl3 5 mM, complete inhibition Coriolopsis trogii
1.10.3.2 KCl 10 mM, 21% inhibition Coriolopsis trogii
1.10.3.2 KI 5 mM, 25% inhibition Coriolopsis trogii
1.10.3.2 MgSO4 10 mM, 6% inhibition Coriolopsis trogii
1.10.3.2 MnSO4 10 mM, 8% inhibition Coriolopsis trogii
1.10.3.2 NaCl 10 mM, 17% inhibition Coriolopsis trogii
1.10.3.2 NaF 0.1 mM, 75% inhibition Coriolopsis trogii
1.10.3.2 NaN3 0.1 mM, complete inhibition Coriolopsis trogii
1.10.3.2 SDS 5 mM, 46% inhibition Coriolopsis trogii
1.10.3.2 ZnSO4 10 mM, 0.5% inhibition Coriolopsis trogii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.00673
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 0.00732
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 0.302
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 0.552
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ contains a type III binuclear Cu2+ center and a type I Cu2+ center Coriolopsis trogii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 54000
-
 gel filtration Coriolopsis trogii

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Coriolopsis trogii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Coriolopsis trogii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 9.1
-
 substrate: poyrogallol, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 13.2
-
 substrate: catechol, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 15.8
-
 substrate: syringaldazine, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 16.7
-
 substrate: guaiacol, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 23.5
-
 substrate: 2,6-dimethoxyphenol, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 36.9
-
 substrate: hydroquinone, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 39.7
-
 substrate: ferulic acid, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 50.1
-
 substrate: phloroglucinol, pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 148
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0, 25°C Coriolopsis trogii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 catechol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 additional information a high-molecular-weight kraft lignin iss effectively polymerized by the enzyme Coriolopsis trogii ?
-
 ?
1.10.3.2 phloroglucinol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Coriolopsis trogii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 64000, SDS-PAGE Coriolopsis trogii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
-
 Coriolopsis trogii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 80 25°C: 29% of maximal activity, 80°C: about 70% of maximal activity Coriolopsis trogii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
 half-life: 1.6 h Coriolopsis trogii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 21.1
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 35.9
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 50.3
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 260
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.2
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Coriolopsis trogii
1.10.3.2 3
-
 substrate: 2,6-dimethoxyphenol Coriolopsis trogii
1.10.3.2 4
-
 substrate: guaiacol Coriolopsis trogii
1.10.3.2 4.5
-
 substrate: syringaldazine Coriolopsis trogii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4
-
 24 h, 25°C, about 60% loss of activity Coriolopsis trogii
1.10.3.2 6 9 24 h, 25°C, stable Coriolopsis trogii
1.10.3.2 10
-
 24 h, 25°C, about 65% loss of activity Coriolopsis trogii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 38
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 170
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 5360
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
1.10.3.2 35519
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii