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Literature summary extracted from

  • Freire, D.M.; Rivas, M.G.; Dias, A.M.; Lopes, A.T.; Costa, C.; Santos-Silva, T.; Van Doorslaer, S.; Gonzalez, P.J.
    The homopentameric chlorite dismutase from Magnetospirillum sp (2015), J. Inorg. Biochem., 151, 1-9 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.49 enzyme is a homopentamer, with presence of one b-type heme per monomer Magnetospirillum sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.49 chloride weak mixed inhibitor that modifies the EPR signal of as-prepared samples Magnetospirillum sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 0.59
-
 Chlorite pH 6.5, 10°C Magnetospirillum sp.
1.13.11.49 1.7
-
 Chlorite pH 6.0, 10°C Magnetospirillum sp.
1.13.11.49 1.7
-
 Chlorite pH 5.5, 10°C Magnetospirillum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.49 140000
-
 gel filtration Magnetospirillum sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Magnetospirillum sp. A0A0M3KL46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.49 the EPR spectroscopic signature of the as-purified Cld samples is affected by the buffer composition. Potassium phosphate buffer is the only buffer that affects neither the spectral nor the kinetic properties of Cld Magnetospirillum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Magnetospirillum sp. chloride + O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.49 pentamer 5 * 27800, MALDI-TOF Magnetospirillum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.49 10
-
 temperature below 10°C is required to avoid enzyme inactivation due to cofactor bleaching during turnover, and to achieve full substrate consumption Magnetospirillum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 6
-
-
 Magnetospirillum sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.49 heme presence of one b-type heme per monomer Magnetospirillum sp.