Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • da Silva, R.R.; Souto, T.B.; de Oliveira, T.B.; de Oliveira, L.C.; Karcher, D.; Juliano, M.A.; Juliano, L.; de Oliveira, A.H.; Rodrigues, A.; Rosa, J.C.; Cabral, H.
    Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei (2016), J. Ind. Microbiol. Biotechnol., 43, 1059-1069 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.23.21 food industry the peptidase may function as an important alternative enzyme in milk clotting during the preparation of cheese Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.21 Al3+ 16% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 Ba2+ about 64 residual activity at 10 mM Rhizomucor miehei
3.4.23.21 Ca2+ about 44% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 Co2+ 0.3% residual activity at 10 mM; about 36% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 CTAB in the presence of CTAB, 20 and 10 % relative activities are maintained at concentrations of 0.1% (w/v) and 1% (w/v), respectively Rhizomucor miehei
3.4.23.21 dithiothreitol the peptidase shows residual activities of 50 and 40% following incubation with 100 or 150 mM dithiothreitol, respectively Rhizomucor miehei
3.4.23.21 guanidine the enzyme maintains approximately 70 and 60% of proteolytic activity at 100 and 150 mM guanidine, respectively Rhizomucor miehei
3.4.23.21 K+ about 56% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 Mg2+ about 36% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 Mn2+ about 36% residual activity at 10 mM Rhizomucor miehei
3.4.23.21 additional information not inhibited by urea Rhizomucor miehei
3.4.23.21 pepstatin A 40% residual activity at 0.2 mM Rhizomucor miehei
3.4.23.21 SDS the peptidase maintains approximately 50 % of activity in the presence of 0.02 % (w/v) SDS andloses nearly all activity during incubation with 0.08 % (w/v) SDS Rhizomucor miehei
3.4.23.21 Triton X-100 approximately 60% residual activity at 0.2 % (v/v) Triton X-100 Rhizomucor miehei
3.4.23.21 Tween 20 approximately 60% residual activity at 0.2 % (v/v) Tween 20 Rhizomucor miehei
3.4.23.23 dithiothreitol 100 mM, 50% residual activity Rhizomucor miehei
3.4.23.23 pepstatin A 0.2 mM, 40% residual activity Rhizomucor miehei
3.4.23.23 SDS 0.02%, 50% residual activity Rhizomucor miehei
3.4.23.23 Triton X-100 0.2%, 60% residual activity Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.21 0.036
-
 Abz-LSFMAIQ-EDDnp at pH 5.5 and 50°C Rhizomucor miehei
3.4.23.23 0.000036
-
 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.000045
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.00011
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.00015
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.0004
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.0005
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.00085
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.23.23 Al3+ 5 mM, 16% residual activity Rhizomucor miehei
3.4.23.23 Ca2+ 5 mM, 44% residual activity Rhizomucor miehei
3.4.23.23 Co2+ 5 mM, 36% residual activity Rhizomucor miehei
3.4.23.23 Cu2+ 5 mM, 0.3% residual activity Rhizomucor miehei
3.4.23.23 Mn2+ 5 mM, 36% residual activity Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.21 Rhizomucor miehei
-
-
-
3.4.23.23 Rhizomucor miehei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.21 Sephacryl S-100 gel filtration and Sephadex G-50 resin gel filtration Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.23 1057
-
 pH 6.5, 45°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.21 Abz-LSFMAIQ-EDDnp + H2O
-
 Rhizomucor miehei Abz-LSF + MAIQ-EDDnp
-
 ?
3.4.23.21 casein + H2O
-
 Rhizomucor miehei ?
-
 ?
3.4.23.21 milk powder + H2O
-
 Rhizomucor miehei ?
-
 ?
3.4.23.21 additional information the coagulant activity of the peptidase is higher than the proteolytic activity and there is a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine Rhizomucor miehei ?
-
 ?
3.4.23.23 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + MSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + WSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O substrate derived from peptide sequence of kappa-casein Rhizomucor miehei 2-aminobenzoyl-LSF + MAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.23 additional information enzyme shows a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine Rhizomucor miehei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.21 ? x * 37000, SDS-PAGE Rhizomucor miehei
3.4.23.21 ? x * 37102, calculated from amino acid sequence Rhizomucor miehei
3.4.23.23 ? x * 37000, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.4.23.21 aspartic peptidase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.21 55
-
-
 Rhizomucor miehei
3.4.23.23 55
-
-
 Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.21 40 65 the enzyme maintains around 80% activity at 40-50°C. 90 and 80% proteolytic activities are maintained at 60 and 65°C, respectively Rhizomucor miehei
3.4.23.23 60
-
 90% of maximum activity Rhizomucor miehei
3.4.23.23 65
-
 80% of maximum activity Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.21 45
-
 the peptidase is stable at temperatures of up 45°C for 1 h Rhizomucor miehei
3.4.23.23 45
-
 stable a up to, for 60 min Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.23 0.045
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.12
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.15
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.35
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 0.5
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1.1
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.21 5.5
-
-
 Rhizomucor miehei
3.4.23.23 5.5
-
-
 Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.21 4.5 6 more than 80% activity between pH 4.5 and 6.0 Rhizomucor miehei
3.4.23.23 5
-
 80% of maximum activity Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.21 3 5 the peptidase is stable at pH values ranging from 3.0 to 5.0 for 1 h Rhizomucor miehei
3.4.23.23 3 5
-
 Rhizomucor miehei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.23.21 4722
-
 Abz-LSFMAIQ-EDDnp at pH 5.5 and 50°C Rhizomucor miehei
3.4.23.23 700
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1000
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1000
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1091
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1250
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 1294
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
3.4.23.23 4722
-
 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei