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Literature summary extracted from

  • Mohammadi, H.S.; Omidinia, E.
    Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems (2013), J. Chromatogr. B, 929, 11-17 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 recombinant expression of the enzyme in Escherichia coli strain BL21 (DE3) plysS inclusion bodies Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2 recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step, method optimization and evaluation, overview Pseudomonas fluorescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.99.B2 recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.B2 600
-
 purified recombinant enzyme in an optimized aqueous two-phase system containing 14% w/w PEG-1000 and 12% w/w Na2CO3, pH 8.0, 37°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + oxidized phenazine methosulfate
-
 Pseudomonas fluorescens (S)-1-pyrroline-5-carboxylate + reduced phenazine methosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 PRODH
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.B2 37
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.B2 8.5
-
 assay at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD dependent on Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.99.B2 Pseudomonas fluorescens isoelectric focussing
-
 6.97

General Information

EC Number General Information Comment Organism
1.5.99.B2 additional information ProDH is a negatively charged protein Pseudomonas fluorescens