BRENDA - Enzyme Database

Protein/protein interactions in the mammalian heme degradation pathway heme oxygenase-2, cytochrome P450 reductase, and biliverdin reductase

Spencer, A.L.; Bagai, I.; Becker, D.F.; Zuiderweg, E.R.; Ragsdale, S.W.; J. Biol. Chem. 289, 29836-29858 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.6.2.4
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.18
K149
42fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
K169A
22fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
L201A
3fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
R87A
1.3fold increase in Km values for cytochrome P450 reductase
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.18
0.5
-
[reduced cytochrome P450 reductase]
wild-type, pH 8.0, 37°C
Homo sapiens
1.14.14.18
0.7
-
[reduced cytochrome P450 reductase]
wild-type, pH 8.0, 37°C
Homo sapiens
1.14.14.18
0.9
-
[reduced cytochrome P450 reductase]
mutant R87A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
2.3
-
[reduced cytochrome P450 reductase]
mutant L201A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
15.2
-
[reduced cytochrome P450 reductase]
mutant K169A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
21.7
-
[reduced cytochrome P450 reductase]
mutant K149A, pH 8.0, 37°C
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.2.4
68600
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.6.2.4
Homo sapiens
-
-
-
1.14.14.18
Homo sapiens
P09601
-
-
1.14.14.18
Homo sapiens
P30519
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.6.2.4
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.2.4
NADPH + H+ + oxidized cytochrome c
-
742864
Homo sapiens
NADP+ + reduced cytochrome c
-
-
-
?
1.14.14.18
protoheme + [reduced cytochrome P450 reductase] + O2
-
742864
Homo sapiens
biliverdin + Fe2+ + CO + [oxidized cytochrome P450 reductase] + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.6.2.4
FAD
-
Homo sapiens
1.6.2.4
FMN
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.2.4
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.6.2.4
FAD
-
Homo sapiens
1.6.2.4
FMN
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.18
K149
42fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
K169A
22fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
L201A
3fold increase in Km values for cytochrome P450 reductase
Homo sapiens
1.14.14.18
R87A
1.3fold increase in Km values for cytochrome P450 reductase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.18
0.5
-
[reduced cytochrome P450 reductase]
wild-type, pH 8.0, 37°C
Homo sapiens
1.14.14.18
0.7
-
[reduced cytochrome P450 reductase]
wild-type, pH 8.0, 37°C
Homo sapiens
1.14.14.18
0.9
-
[reduced cytochrome P450 reductase]
mutant R87A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
2.3
-
[reduced cytochrome P450 reductase]
mutant L201A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
15.2
-
[reduced cytochrome P450 reductase]
mutant K169A, pH 8.0, 37°C
Homo sapiens
1.14.14.18
21.7
-
[reduced cytochrome P450 reductase]
mutant K149A, pH 8.0, 37°C
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.2.4
68600
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.2.4
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.2.4
NADPH + H+ + oxidized cytochrome c
-
742864
Homo sapiens
NADP+ + reduced cytochrome c
-
-
-
?
1.14.14.18
protoheme + [reduced cytochrome P450 reductase] + O2
-
742864
Homo sapiens
biliverdin + Fe2+ + CO + [oxidized cytochrome P450 reductase] + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.14.18
physiological function
Hmox2 and cytochrome P450 reductase form a dynamic ensemble of complexes that precede formation of the productive electron transfer complex. Specific residues, including Leu201, near the heme face of Hmox2 are affected by the addition of cytochrome P450 reductase. Hmox2 and biliverdin reductase form a very weak complex, and biliverdin reductase binds Hmox2 weakly
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.18
physiological function
Hmox2 and cytochrome P450 reductase form a dynamic ensemble of complexes that precede formation of the productive electron transfer complex. Specific residues, including Leu201, near the heme face of Hmox2 are affected by the addition of cytochrome P450 reductase. Hmox2 and biliverdin reductase form a very weak complex, and biliverdin reductase binds Hmox2 weakly
Homo sapiens