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Literature summary extracted from

  • McTernan, P.M.; Chandrayan, S.K.; Wu, C.H.; Vaccaro, B.J.; Lancaster, W.A.; Yang, Q.; Fu, D.; Hura, G.L.; Tainer, J.A.; Adams, M.W.
    Intact functional fourteen-subunit respiratory membrane-bound [NiFe]-hydrogenase complex of the hyperthermophilic archaeon Pyrococcus furiosus (2014), J. Biol. Chem., 289, 19364-19372 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 membrane
-
 Pyrococcus furiosus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 310000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Pyrococcus furiosus
-
 reduced ferredoxin + H+
-
 ?
1.12.7.2 H2 + oxidized ferredoxin Pyrococcus furiosus COM1
-
 reduced ferredoxin + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Pyrococcus furiosus
-
-
-
1.12.7.2 Pyrococcus furiosus COM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 Ni-NTA column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + methyl viologen
-
 Pyrococcus furiosus reduced methyl viologen + H+
-
 ?
1.12.7.2 H2 + methyl viologen
-
 Pyrococcus furiosus COM1 reduced methyl viologen + H+
-
 ?
1.12.7.2 H2 + oxidized ferredoxin
-
 Pyrococcus furiosus reduced ferredoxin + H+
-
 ?
1.12.7.2 H2 + oxidized ferredoxin
-
 Pyrococcus furiosus COM1 reduced ferredoxin + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 ? x * 298000, calculated from amino acid sequence Pyrococcus furiosus
1.12.7.2 ? x * 310000, small angle X-ray scattering Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 MDH
-
 Pyrococcus furiosus
1.12.7.2 membrane-bound hydrogenase
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 90
-
 the membrane-bound and purified enzymes show half-lives at 90°C of 1 and 25 h, respectively Pyrococcus furiosus