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Literature summary extracted from

  • Carlson, B.L.; Ballister, E.R.; Skordalakes, E.; King, D.S.; Breidenbach, M.A.; Gilmore, S.A.; Berger, J.M.; Bertozzi, C.R.
    Function and structure of a prokaryotic formylglycine-generating enzyme (2008), J. Biol. Chem., 283, 20117-20125 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.7 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
1.8.3.7 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.3.7 vapor diffusion method, using 100 mM Tris, 2.4 M ammonium formate, 0.3% (w/v) beta-octylglucoside, 3.2% (v/v) 2-butanol, pH 8.0 Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.7 C263S inactive Mycobacterium tuberculosis
1.8.3.7 C263S inactive Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.7 Ca2+ the enzyme requires a Ca2+ ion Streptomyces coelicolor
1.8.3.7 additional information the enzyme does not require a divalent cation Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.7 Mycobacterium tuberculosis
-
-
-
1.8.3.7 Mycobacterium tuberculosis H37Rv
-
-
-
1.8.3.7 Streptomyces coelicolor
-
-
-
1.8.3.7 Streptomyces coelicolor ATCC BAA-471
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.7 amylose resin column chromatography Streptomyces coelicolor
1.8.3.7 HisTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.7 LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Streptomyces coelicolor leucyl-formylglycyl-SPSRGSLFTGR + ?
-
 ?
1.8.3.7 LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Streptomyces coelicolor ATCC BAA-471 leucyl-formylglycyl-SPSRGSLFTGR + ?
-
 ?
1.8.3.7 LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Mycobacterium tuberculosis leucyl-formylglycyl-SPSRGSLFTGR + sulfide + oxidized dithiothreitol + H2O
-
 ?
1.8.3.7 LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Mycobacterium tuberculosis H37Rv leucyl-formylglycyl-SPSRGSLFTGR + sulfide + oxidized dithiothreitol + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.7 FGE
-
 Mycobacterium tuberculosis
1.8.3.7 FGE
-
 Streptomyces coelicolor