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Literature summary extracted from

  • Pojer, F.; Kahlich, R.; Kammerer, B.; Li, S.M.; Heide, L.
    CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis (2003), J. Biol. Chem., 278, 30661-30668 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.83 additional information the enzyme is not stimulated by addition of 2-oxoglutarate Streptomyces roseochromogenus
1.13.12.23 additional information the enzyme is not stimulated by addition of 2-oxoglutarate Streptomyces roseochromogenus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.83 expressed in Escherichia coli BL21(DE3) cells Streptomyces roseochromogenus
1.13.12.23 expressed in Escherichia coli BL21(DE3) cells Streptomyces roseochromogenus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.83 Fe2+ a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity Streptomyces roseochromogenus
1.13.12.23 Fe2+ a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity Streptomyces roseochromogenus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.83 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 Streptomyces roseochromogenus
-
 3-dimethylallyl-4-hydroxymandelate + CO2
-
 ?
1.13.11.83 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 Streptomyces roseochromogenus DS 12976
-
 3-dimethylallyl-4-hydroxymandelate + CO2
-
 ?
1.13.12.23 3-dimethylallyl-4-hydroxymandelate + O2 Streptomyces roseochromogenus
-
 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?
1.13.12.23 3-dimethylallyl-4-hydroxymandelate + O2 Streptomyces roseochromogenus DS 12976
-
 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.83 Streptomyces roseochromogenus Q8GHB1
-
-
1.13.11.83 Streptomyces roseochromogenus DS 12976 Q8GHB1
-
-
1.13.12.23 Streptomyces roseochromogenus Q8GHB1
-
-
1.13.12.23 Streptomyces roseochromogenus DS 12976 Q8GHB1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.83 glutathione-Sepharose column chromatography Streptomyces roseochromogenus
1.13.12.23 glutathione-Sepharose column chromatography Streptomyces roseochromogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.83 3-dimethylallyl-4-hydroxyphenylpyruvate + O2
-
 Streptomyces roseochromogenus 3-dimethylallyl-4-hydroxymandelate + CO2
-
 ?
1.13.11.83 3-dimethylallyl-4-hydroxyphenylpyruvate + O2
-
 Streptomyces roseochromogenus DS 12976 3-dimethylallyl-4-hydroxymandelate + CO2
-
 ?
1.13.12.23 3-dimethylallyl-4-hydroxymandelate + O2
-
 Streptomyces roseochromogenus 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?
1.13.12.23 3-dimethylallyl-4-hydroxymandelate + O2
-
 Streptomyces roseochromogenus DS 12976 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.83 ? x * 30500, calculated from amino acid sequence Streptomyces roseochromogenus
1.13.12.23 ? x * 30500, calculated from amino acid sequence Streptomyces roseochromogenus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.83 CloR
-
 Streptomyces roseochromogenus
1.13.12.23 CloR
-
 Streptomyces roseochromogenus