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Literature summary extracted from

  • Takahashi, S.; Shimada, K.; Nozawa, S.; Goto, M.; Abe, K.; Kera, Y.
    Possible role of a histidine residue in the substrate specificity of yeast D-aspartate oxidase (2016), J. Biochem., 159, 371-378 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expressed in Escherichia coli BL21(DE3) cells Vanrija humicola

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.1 H56A the mutant exhibits no significant activity toward acidic D-amino acids Vanrija humicola
1.4.3.1 H56A the mutant gains the ability to utilize neutral D-amino acids as substrates, such as D-methionine, D-phenylalanine and D-glutamine Vanrija humicola
1.4.3.1 H56D the mutant exhibits no significant activity toward acidic D-amino acids Vanrija humicola
1.4.3.1 H56F the mutant exhibits no significant activity toward acidic D-amino acids Vanrija humicola
1.4.3.1 H56K the mutant exhibits no significant activity toward acidic D-amino acids Vanrija humicola
1.4.3.1 H56N the mutant exhibits no significant activity toward acidic D-amino acids Vanrija humicola
1.4.3.1 H56N the mutant gains the ability to utilize neutral D-amino acids as substrates, such as D-methionine, D-phenylalanine and D-glutamine Vanrija humicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.1 D-malate 87.8% residual activity at 10 mM Vanrija humicola
1.4.3.1 malonate 62.1% residual activity at 10 mM Vanrija humicola
1.4.3.1 additional information not inhibited by 10 mM meso-tartrate, benzoate, anthranilate, and crotonate Vanrija humicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.1 2.5
-
 D-Asp wild type enzyme, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 8.5
-
 D-Met mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 8.8
-
 D-Leu mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 14.3
-
 D-Phe mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 21
-
 D-Gln mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 31.8
-
 D-Met mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 34.8
-
 D-Phe mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 39.4
-
 D-Gln mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 83.4
-
 D-Asn mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 106
-
 D-Glu wild type enzyme, at pH 7.5 and 37°C Vanrija humicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.1 D-Asp + H2O + O2 Vanrija humicola the enzyme shows high activity only with D-aspartate oxaloacetate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Vanrija humicola Q75WF1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 D-Asn + H2O + O2 low activity Vanrija humicola 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.1 D-Asn + H2O + O2 substrate for mutant enzyme H56N Vanrija humicola ? + NH3 + H2O2
-
 ?
1.4.3.1 D-Asp + H2O + O2 the enzyme shows high activity only with D-aspartate Vanrija humicola oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.1 D-Gln + H2O + O2 substrate for mutant enzymes H56A and H56N Vanrija humicola ? + NH3 + H2O2
-
 ?
1.4.3.1 D-Glu + H2O + O2 low activity Vanrija humicola 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 D-Leu + H2O + O2 substrate for mutant enzyme H56N Vanrija humicola ? + NH3 + H2O2
-
 ?
1.4.3.1 D-Met + H2O + O2 substrate for mutant enzymes H56A and H56N Vanrija humicola 4-(methylsulfonyl)-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.1 D-Phe + H2O + O2 substrate for mutant enzymes H56A and H56N Vanrija humicola ? + NH3 + H2O2
-
 ?
1.4.3.1 additional information no activity with D-Ala, D-Ser, D-Pro, D-Val, D-Thr, D-Ile, D-Leu, D-Gln, D-Met, D-Phe, D-Tyr, D-Trp, D-Lys, D-His, D-Arg, and Gly Vanrija humicola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.1 ? x * 40000, SDS-PAGE Vanrija humicola

Synonyms

EC Number Synonyms Comment Organism
1.4.3.1 DDO
-
 Vanrija humicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.1 0.7
-
 D-Leu mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 2.2
-
 D-Gln mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 2.6
-
 D-Glu wild type enzyme, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 3.1
-
 D-Phe mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 3.9
-
 D-Asn mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 4
-
 D-Met mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 4.1
-
 D-Phe mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 4.5
-
 D-Gln mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 11.5
-
 D-Met mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 74.5
-
 D-Asp wild type enzyme, at pH 7.5 and 37°C Vanrija humicola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.1 0.024
-
 D-Glu wild type enzyme, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.047
-
 D-Asn mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.055
-
 D-Gln mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.074
-
 D-Leu mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.119
-
 D-Phe mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.125
-
 D-Met mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.213
-
 D-Gln mutant enzyme H56N, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 0.218
-
 D-Phe mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 1.35
-
 D-Met mutant enzyme H56A, at pH 7.5 and 37°C Vanrija humicola
1.4.3.1 29.8
-
 D-Asp wild type enzyme, at pH 7.5 and 37°C Vanrija humicola