BRENDA - Enzyme Database show

Identification and validation of aspartic acid semialdehyde dehydrogenase as a new anti-Mycobacterium tuberculosis target

Meng, J.; Yang, Y.; Xiao, C.; Guan, Y.; Hao, X.; Deng, Q.; Lu, Z.; Int. J. Mol. Sci. 16, 23572-23586 (2015)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.11
additional information
construction of an gene asadh promoter-replacement mutant Mycobacterium tuberculosis strain, designated MTB::asadh, in which the asadh gene expression is regulated by pristinamycin. Bacterial growth is survival of MTB::asadh in host cells is completely inhibited in the absence of the inducer pristinamycin. The growth of the mutant is rigorously dependent on the presence of the inducer in the medium. The starved mutant exhibits a marked reduction (approximately 80%) in the cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview. With the addition of pristinamycin, the cell wall contents and morphology are recovered in a similar manner to those of the wild-type strain. The starved mutant also exhibits almost no pathogenicity in an in vitro model of infection using mouse macrophage J774A.1 cells. The mutant shows a concentration-dependent recovery of pathogenicity with the addition of the inducer
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Mycobacterium tuberculosis
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Mycobacterium tuberculosis H37Rv
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Mycobacterium tuberculosis
-
-
-
1.2.1.11
Mycobacterium tuberculosis H37Rv
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
742704
Mycobacterium tuberculosis
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
742704
Mycobacterium tuberculosis H37Rv
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Mycobacterium tuberculosis
1.2.1.11
NADPH
-
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Mycobacterium tuberculosis
1.2.1.11
NADPH
-
Mycobacterium tuberculosis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.11
additional information
construction of an gene asadh promoter-replacement mutant Mycobacterium tuberculosis strain, designated MTB::asadh, in which the asadh gene expression is regulated by pristinamycin. Bacterial growth is survival of MTB::asadh in host cells is completely inhibited in the absence of the inducer pristinamycin. The growth of the mutant is rigorously dependent on the presence of the inducer in the medium. The starved mutant exhibits a marked reduction (approximately 80%) in the cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview. With the addition of pristinamycin, the cell wall contents and morphology are recovered in a similar manner to those of the wild-type strain. The starved mutant also exhibits almost no pathogenicity in an in vitro model of infection using mouse macrophage J774A.1 cells. The mutant shows a concentration-dependent recovery of pathogenicity with the addition of the inducer
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Mycobacterium tuberculosis
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Mycobacterium tuberculosis H37Rv
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
742704
Mycobacterium tuberculosis
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
742704
Mycobacterium tuberculosis H37Rv
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
General Information
EC Number
General Information
Commentary
Organism
1.2.1.11
malfunction
enzyme deficiency or inhibition of enzyme activity leads to 80% reduced cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview
Mycobacterium tuberculosis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.2.1.11
malfunction
enzyme deficiency or inhibition of enzyme activity leads to 80% reduced cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview
Mycobacterium tuberculosis