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Literature summary extracted from

  • Gupta, A.; Kesherwani, M.; Velmurugan, D.; Tripathi, T.
    Fasciola gigantica thioredoxin glutathione reductase Biochemical properties and structural modeling (2016), Int. J. Biol. Macromol., 89, 152-160 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.B1 expression in Escherichia coli Fasciola gigantica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.B1 modeled structure is a functional dimer, and contains a Grx domain (residues 21–83), a FAD/NADPH-binding domain or TrxR domain (residues 109–450) and a dimerization domain (residues 470–598) Fasciola gigantica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.00434
-
5,5'-dithiobis(2-nitrobenzoic acid) pH 7.5, 25°C Fasciola gigantica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.B1 130000
-
gel filtration Fasciola gigantica

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Fasciola gigantica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Fasciola gigantica 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.B1 glutathione disulfide + NADPH + H+ enzyme displays hysteretic behavior Fasciola gigantica 2 glutathione + NADP+
-
?
1.8.1.B1 oxidized insulin + NADPH + H+
-
Fasciola gigantica reduced insulin + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.B1 dimer 2 * 66000, calculated and SDS-PAGE Fasciola gigantica

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.B1 FAD the flavin ring of FAD cofactor binds in a hydrophobic cleft formed by Cys154, Val157, Gly158, Cys159, Pro572, Phe474, and Pro443 Fasciola gigantica
1.8.1.B1 NADPH nicotinamide moiety binds up in the hydrophobic cleft formed by Val348, Ile266, Pro264,Val292, Met315, Val316, and Pro349, etc. and the diphosphate forms H-bond with Ile391, Ser318, and Arg317. The ribose sugar formes H-bonds with Arg262, Arg322, and Tyr296 Fasciola gigantica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.B1 Fasciola gigantica calculated
-
6.3