EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.B1 | expression in Escherichia coli | Fasciola gigantica |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.1.B1 | modeled structure is a functional dimer, and contains a Grx domain (residues 2183), a FAD/NADPH-binding domain or TrxR domain (residues 109450) and a dimerization domain (residues 470598) | Fasciola gigantica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.B1 | 0.00434 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | pH 7.5, 25°C | Fasciola gigantica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.B1 | 130000 | - |
gel filtration | Fasciola gigantica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.B1 | Fasciola gigantica | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.B1 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Fasciola gigantica | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.B1 | glutathione disulfide + NADPH + H+ | enzyme displays hysteretic behavior | Fasciola gigantica | 2 glutathione + NADP+ | - |
? | |
1.8.1.B1 | oxidized insulin + NADPH + H+ | - |
Fasciola gigantica | reduced insulin + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.B1 | dimer | 2 * 66000, calculated and SDS-PAGE | Fasciola gigantica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.B1 | FAD | the flavin ring of FAD cofactor binds in a hydrophobic cleft formed by Cys154, Val157, Gly158, Cys159, Pro572, Phe474, and Pro443 | Fasciola gigantica | |
1.8.1.B1 | NADPH | nicotinamide moiety binds up in the hydrophobic cleft formed by Val348, Ile266, Pro264,Val292, Met315, Val316, and Pro349, etc. and the diphosphate forms H-bond with Ile391, Ser318, and Arg317. The ribose sugar formes H-bonds with Arg262, Arg322, and Tyr296 | Fasciola gigantica |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.8.1.B1 | Fasciola gigantica | calculated | - |
6.3 |