EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.14 | sodium carbonate | 50 mM, 125% of initial activity | Streptomyces griseosporeus | |
1.11.1.14 | tetraacetylethylenediamine | 25 mM, 121% of initial activity | Streptomyces griseosporeus | |
1.11.1.14 | Triton X-100 | 5% w/v, 123% of initial activity | Streptomyces griseosporeus |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.11.1.14 | degradation | enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C | Streptomyces griseosporeus |
EC Number | General Stability | Organism |
---|---|---|
1.11.1.19 | the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively. | Streptomyces griseosporeus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.14 | SDS | 1% w/v, 72% residual activity | Streptomyces griseosporeus | |
1.11.1.14 | sodium perborate | 5% w/v, 71% residual activity | Streptomyces griseosporeus | |
1.11.1.14 | Urea | 1% w/v, 85% residual activity | Streptomyces griseosporeus | |
1.11.1.19 | Ba2+ | about 85% inhibition at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | Cd2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | Co2+ | about 56% inhibition at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | Hg2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | potassium cyanide | 13% inhibition at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | Sodium azide | 9% inhibition at 1 mM | Streptomyces griseosporeus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | 0.11 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.14 | 0.46 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.19 | 0.112 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
1.11.1.19 | 0.458 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.14 | extracellular | - |
Streptomyces griseosporeus | - |
- |
1.11.1.19 | extracellular | - |
Streptomyces griseosporeus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.19 | Ca2+ | about 116% activity at 1 mM | Streptomyces griseosporeus | |
1.11.1.19 | Mn2+ | about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme | Streptomyces griseosporeus | |
1.11.1.19 | additional information | Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity | Streptomyces griseosporeus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.14 | Streptomyces griseosporeus | - |
- |
- |
1.11.1.14 | Streptomyces griseosporeus SN9 | - |
- |
- |
1.11.1.19 | Streptomyces griseosporeus | - |
- |
- |
1.11.1.19 | Streptomyces griseosporeus SN9 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.14 | purification from culture supernatant | Streptomyces griseosporeus |
1.11.1.19 | OH-40 column chromatography and UNO Q-6 column chromatography | Streptomyces griseosporeus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 5.75 | - |
substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication | Streptomyces griseosporeus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.14 | 2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
1.11.1.14 | 2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
1.11.1.14 | 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | 92% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | 92% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
1.11.1.14 | 2,4,6-trichlorophenol + H2O2 | 63% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | 2,4,6-trichlorophenol + H2O2 | 63% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
1.11.1.14 | 2,4-dichlorophenol + H2O2 | best substrate | Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | 4-chlorophenol + H2O2 | 45% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | humic acid + H2O2 | - |
Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | humic acid + H2O2 | - |
Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
1.11.1.14 | o-dianisidine + H2O2 | 76% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
1.11.1.14 | o-dianisidine + H2O2 | 76% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
1.11.1.19 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | about 92% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | 2,4,6-trichlorophenol + H2O2 + H+ | about 63% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | 2,4-dichlorophenol + H2O2 + H+ | 100% activity | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | 4-chlorophenol + H2O2 + H+ | about 45% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | Acid Blue 158 + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | Cibacet Brilliant Blue BG + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | guaiacol + H2O2 + H+ | about 27% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | additional information | no activity with commercial humic acid | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | o-dianisidine + H2O2 + H+ | about 76% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | pyrogallol + H2O2 + H+ | about 75% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
1.11.1.19 | Remazol Brilliant Blue Reactif + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.14 | ? | x * 43000, SDS-PAGE | Streptomyces griseosporeus |
1.11.1.19 | monomer | 1 * 43000, SDS-PAGE | Streptomyces griseosporeus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.19 | LiP-SN | - |
Streptomyces griseosporeus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 60 | - |
- |
Streptomyces griseosporeus |
1.11.1.19 | 65 | - |
- |
Streptomyces griseosporeus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 60 | - |
the half-life at 60°C is 120 min | Streptomyces griseosporeus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | 0.06 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.14 | 1.16 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.19 | 0.0635 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
1.11.1.19 | 1.17 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 8.5 | - |
- |
Streptomyces griseosporeus |
1.11.1.19 | 8.5 | - |
- |
Streptomyces griseosporeus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 7 | 10 | the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C | Streptomyces griseosporeus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.14 | heme | spectrum shows a heme peak at 409 nm | Streptomyces griseosporeus | |
1.11.1.19 | heme | - |
Streptomyces griseosporeus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | 0.57 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.14 | 2.53 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.11.1.19 | 0.567 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
1.11.1.19 | 2.54 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |