EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.3.1 | expression in Escherichia coli strain BL21 | Bacillus sp. BSD-8 |
EC Number | General Stability | Organism |
---|---|---|
1.5.3.1 | modified technique is successfully established for efficiently improving the stability of sarcosine oxidase via poly-Lys covalent binding onto enzymatic surface. Compared to the free enzyme, higher pH stability and thermostability of modified sarcosine oxidase is achieved with a series of characterization analysis. It is owing to that the loosening of three-dimensional configuration can be restricted by the enzymatic surface protection with poly-Lys and subsequently maintained a more stable enzyme structure. The changed microenvironment around the enzyme in themodification process can also contribute to the better stability | Bacillus sp. BSD-8 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 47.94 | - |
sarcosine | 37°C, pH 8.0, poly-lysine modified enzyme | Bacillus sp. BSD-8 | |
1.5.3.1 | 141.65 | - |
sarcosine | 37°C, pH 8.0, soluble enzyme | Bacillus sp. BSD-8 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 43800 | - |
soluble enzyme, mass spectrum analysis | Bacillus sp. BSD-8 |
1.5.3.1 | 48000 | - |
and 51700, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis | Bacillus sp. BSD-8 |
1.5.3.1 | 51700 | - |
and 48000, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis | Bacillus sp. BSD-8 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + H2O + O2 | Bacillus sp. BSD-8 | - |
glycine + formaldehyde + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.3.1 | Bacillus sp. BSD-8 | Q6ITC6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.3.1 | - |
Bacillus sp. BSD-8 |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.3.1 | 37°C, pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% | Bacillus sp. BSD-8 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + H2O + O2 | - |
Bacillus sp. BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 37 | - |
pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% | Bacillus sp. BSD-8 |
1.5.3.1 | 60 | - |
10 min, residual activity of soluble enzyme is 2.3%, residual activity poly-lysine modified enzymeis 74.7% | Bacillus sp. BSD-8 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 5 | 10 | 37°C, 2.5 h, more than 90% of initial activity of poly-lysine modified enzyme is maintained | Bacillus sp. BSD-8 |