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Literature summary extracted from

  • Sasoni, N.; Iglesias, A.A.; Guerrero, S.A.; Arias, D.G.
    Functional thioredoxin reductase from pathogenic and free-living Leptospira spp. (2016), Free Radic. Biol. Med., 97, 1-13 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0021
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira interrogans serovar Copenhageni
1.8.1.9 0.0022
-
 NADPH pH 7.0, 30°C Leptospira interrogans serovar Copenhageni
1.8.1.9 0.0067
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira biflexa serovar Patoc
1.8.1.9 0.007
-
 NADPH pH 7.0, 30°C Leptospira biflexa serovar Patoc

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 70000
-
 gel filtration Leptospira interrogans serovar Copenhageni
1.8.1.9 70000
-
 gel filtration Leptospira biflexa serovar Patoc

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Leptospira biflexa serovar Patoc B0SKV7
-
-
1.8.1.9 Leptospira biflexa serovar Patoc ATCC 23582 B0SKV7
-
-
1.8.1.9 Leptospira interrogans serovar Copenhageni Q72SB4
-
-
1.8.1.9 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 Q72SB4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Leptospira biflexa serovar Patoc 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Leptospira biflexa serovar Patoc ATCC 23582 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 bis-gamma-glutamyl cystine + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni gamma-glutamyl cystine + NADP+
-
 ?
1.8.1.9 bis-gamma-glutamyl cystine + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 gamma-glutamyl cystine + NADP+
-
 ?
1.8.1.9 cystine + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni 2 cysteine + NADP+
-
 ?
1.8.1.9 glutathione disulfide + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni glutathione + NADP+
-
 ?
1.8.1.9 glutathione disulfide + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 glutathione + NADP+
-
 ?
1.8.1.9 lipoamide disulfide + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni lipoamide + NADP+
-
 ?
1.8.1.9 additional information no substrates: ferredoxin, flavodoxin Leptospira interrogans serovar Copenhageni ?
-
 ?
1.8.1.9 additional information no substrates: ferredoxin, flavodoxin Leptospira biflexa serovar Patoc ?
-
 ?
1.8.1.9 additional information no substrates: ferredoxin, flavodoxin Leptospira interrogans serovar Copenhageni Fiocruz L1-130 ?
-
 ?
1.8.1.9 additional information no substrates: ferredoxin, flavodoxin Leptospira biflexa serovar Patoc ATCC 23582 ?
-
 ?
1.8.1.9 peroxiredoxin + NADPH + H+
-
 Leptospira interrogans serovar Copenhageni peroxiredoxin disulfide + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH
-
 Leptospira interrogans serovar Copenhageni thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH
-
 Leptospira biflexa serovar Patoc thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH
-
 Leptospira biflexa serovar Patoc ATCC 23582 thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 34000, calculated Leptospira interrogans serovar Copenhageni
1.8.1.9 dimer 2 * 34000, calculated Leptospira biflexa serovar Patoc

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrxB
-
 Leptospira interrogans serovar Copenhageni
1.8.1.9 TrxB
-
 Leptospira biflexa serovar Patoc
1.8.1.9 TrxR1
-
 Leptospira biflexa serovar Patoc

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 4.01
-
 NADPH pH 7.0, 30°C Leptospira biflexa serovar Patoc
1.8.1.9 6
-
 NADPH pH 7.0, 30°C Leptospira interrogans serovar Copenhageni
1.8.1.9 7.7
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira interrogans serovar Copenhageni
1.8.1.9 13.3
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira biflexa serovar Patoc

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.9 7
-
-
 Leptospira interrogans serovar Copenhageni
1.8.1.9 7
-
-
 Leptospira biflexa serovar Patoc

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
 Leptospira interrogans serovar Copenhageni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 590
-
 NADPH pH 7.0, 30°C Leptospira biflexa serovar Patoc
1.8.1.9 1900
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira biflexa serovar Patoc
1.8.1.9 2800
-
 NADPH pH 7.0, 30°C Leptospira interrogans serovar Copenhageni
1.8.1.9 3700
-
 thioredoxin disulfide pH 7.0, 30°C Leptospira interrogans serovar Copenhageni