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Literature summary extracted from

  • Samhan-Arias, A.K.; Gutierrez-Merino, C.
    Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin sensitivity to nitric oxide and peroxynitrite (2014), Free Radic. Biol. Med., 73, 174-189 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 apocynin 90.1% inhibition at 0.2 mM Sus scrofa
1.6.2.2 nitric oxide
-
 Sus scrofa
1.6.2.2 peroxynitrite
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0028
-
 NADH at pH 7.4 and 37°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 microsome
-
 Sus scrofa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.2 NADH + ferricytochrome b5 Sus scrofa
-
 NAD+ + H+ + ferrocytochrome b5
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 ammonium sulfate precipitation, DE-52 cellulose column chromatography, hydroxylapatite column chromatography, and NAD+-agarose column chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 brain
-
 Sus scrofa
-
1.6.2.2 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 NADH + ferricyanide
-
 Sus scrofa NAD+ + H+ + ferrocyanide
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Sus scrofa NAD+ + H+ + ferrocytochrome b5
-
 ?
1.6.2.2 NADH + oxidized cytochrome c
-
 Sus scrofa NAD+ + H+ + reduced cytochrome c
-
 ?
1.6.2.2 NADH + oxidized nitroblue tetrazolium
-
 Sus scrofa NAD+ + H+ + reduced nitroblue tetrazolium
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 33000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.6.2.2 cb5r
-
 Sus scrofa
1.6.2.2 NADH-cytochrome b5 reductase
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.2 0.07
-
 apocynin at pH 7.4 and 37°C Sus scrofa