BRENDA - Enzyme Database

Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin sensitivity to nitric oxide and peroxynitrite

Samhan-Arias, A.K.; Gutierrez-Merino, C.; Free Radic. Biol. Med. 73, 174-189 (2014)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.2.2
apocynin
90.1% inhibition at 0.2 mM
Sus scrofa
1.6.2.2
nitric oxide
-
Sus scrofa
1.6.2.2
peroxynitrite
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.2.2
0.0028
-
NADH
at pH 7.4 and 37°C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.2.2
microsome
-
Sus scrofa
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.6.2.2
NADH + ferricytochrome b5
Sus scrofa
-
NAD+ + H+ + ferrocytochrome b5
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.6.2.2
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.6.2.2
ammonium sulfate precipitation, DE-52 cellulose column chromatography, hydroxylapatite column chromatography, and NAD+-agarose column chromatography
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.6.2.2
brain
-
Sus scrofa
-
1.6.2.2
liver
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.2.2
NADH + ferricyanide
-
742559
Sus scrofa
NAD+ + H+ + ferrocyanide
-
-
-
?
1.6.2.2
NADH + ferricytochrome b5
-
742559
Sus scrofa
NAD+ + H+ + ferrocytochrome b5
-
-
-
?
1.6.2.2
NADH + oxidized cytochrome c
-
742559
Sus scrofa
NAD+ + H+ + reduced cytochrome c
-
-
-
?
1.6.2.2
NADH + oxidized nitroblue tetrazolium
-
742559
Sus scrofa
NAD+ + H+ + reduced nitroblue tetrazolium
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.6.2.2
?
x * 33000, SDS-PAGE
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.6.2.2
0.07
-
apocynin
at pH 7.4 and 37°C
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.2.2
apocynin
90.1% inhibition at 0.2 mM
Sus scrofa
1.6.2.2
nitric oxide
-
Sus scrofa
1.6.2.2
peroxynitrite
-
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.6.2.2
0.07
-
apocynin
at pH 7.4 and 37°C
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.2.2
0.0028
-
NADH
at pH 7.4 and 37°C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.2.2
microsome
-
Sus scrofa
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.6.2.2
NADH + ferricytochrome b5
Sus scrofa
-
NAD+ + H+ + ferrocytochrome b5
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.2.2
ammonium sulfate precipitation, DE-52 cellulose column chromatography, hydroxylapatite column chromatography, and NAD+-agarose column chromatography
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.6.2.2
brain
-
Sus scrofa
-
1.6.2.2
liver
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.2.2
NADH + ferricyanide
-
742559
Sus scrofa
NAD+ + H+ + ferrocyanide
-
-
-
?
1.6.2.2
NADH + ferricytochrome b5
-
742559
Sus scrofa
NAD+ + H+ + ferrocytochrome b5
-
-
-
?
1.6.2.2
NADH + oxidized cytochrome c
-
742559
Sus scrofa
NAD+ + H+ + reduced cytochrome c
-
-
-
?
1.6.2.2
NADH + oxidized nitroblue tetrazolium
-
742559
Sus scrofa
NAD+ + H+ + reduced nitroblue tetrazolium
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.6.2.2
?
x * 33000, SDS-PAGE
Sus scrofa