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Literature summary extracted from
- Phenylalanine ammonia-lyase, flavanone 3beta-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits (2014), Food Chem., 153, 130-133 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods | Rubus plicatus |
| 1.14.11.9 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods | Rubus idaeus |
| 1.14.11.9 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods | Ribes nigrum |
| 1.14.11.9 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods | Fragaria x ananassa |
| 1.14.11.9 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods | Ribes rubrum |
| 1.14.20.6 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Rubus plicatus |
| 1.14.20.6 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Rubus idaeus |
| 1.14.20.6 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes nigrum |
| 1.14.20.6 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Fragaria x ananassa |
| 1.14.20.6 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes rubrum |
| 4.3.1.24 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Rubus plicatus |
| 4.3.1.24 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Fragaria x ananassa |
| 4.3.1.24 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes nigrum |
| 4.3.1.24 | analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes rubrum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Rubus idaeus | - |
dihydrokaempferol + succinate + CO2 | - |
? |  |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Fragaria x ananassa | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Vaccinium myrtillus | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Ribes rubrum | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Ribes nigrum | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | Fragaria x ananassa | Q66ME9 | - |
- |
| 1.14.11.9 | Ribes nigrum | - |
- |
- |
| 1.14.11.9 | Ribes nigrum | A0A0C7DVW3 | - |
- |
| 1.14.11.9 | Ribes rubrum | - |
- |
- |
| 1.14.11.9 | Ribes rubrum | A0A172MJX5 | - |
- |
| 1.14.11.9 | Rubus idaeus | - |
- |
- |
| 1.14.11.9 | Rubus plicatus | - |
- |
- |
| 1.14.11.9 | Vaccinium myrtillus | Q8H249 | - |
- |
| 1.14.20.6 | Fragaria x ananassa | Q07DT9 | - |
- |
| 1.14.20.6 | Ribes nigrum | - |
- |
- |
| 1.14.20.6 | Ribes rubrum | - |
- |
- |
| 1.14.20.6 | Rubus idaeus | - |
- |
- |
| 1.14.20.6 | Rubus plicatus | - |
- |
- |
| 4.3.1.24 | Fragaria x ananassa | - |
- |
- |
| 4.3.1.24 | Ribes nigrum | - |
- |
- |
| 4.3.1.24 | Ribes rubrum | - |
- |
- |
| 4.3.1.24 | Rubus plicatus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | berry | - |
Rubus idaeus | - |
| 1.14.11.9 | berry | - |
Fragaria x ananassa | - |
| 1.14.11.9 | berry | - |
Vaccinium myrtillus | - |
| 1.14.11.9 | berry | - |
Ribes rubrum | - |
| 1.14.11.9 | berry | - |
Ribes nigrum | - |
| 1.14.11.9 | fruit | - |
Rubus plicatus | - |
| 1.14.11.9 | fruit | - |
Rubus idaeus | - |
| 1.14.11.9 | fruit | - |
Ribes nigrum | - |
| 1.14.11.9 | fruit | - |
Fragaria x ananassa | - |
| 1.14.11.9 | fruit | - |
Ribes rubrum | - |
| 1.14.11.9 | fruit | - |
Vaccinium myrtillus | - |
| 1.14.20.6 | fruit | - |
Rubus plicatus | - |
| 1.14.20.6 | fruit | - |
Rubus idaeus | - |
| 1.14.20.6 | fruit | - |
Ribes nigrum | - |
| 1.14.20.6 | fruit | - |
Fragaria x ananassa | - |
| 1.14.20.6 | fruit | - |
Ribes rubrum | - |
| 4.3.1.24 | fruit | - |
Rubus plicatus | - |
| 4.3.1.24 | fruit | - |
Fragaria x ananassa | - |
| 4.3.1.24 | fruit | - |
Ribes nigrum | - |
| 4.3.1.24 | fruit | - |
Ribes rubrum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.9 | additional information | development of a reverse phase (C18) HPLC method (with elutant ethylacetate) for the determination of flavanone 3beta-hydroxylase and other enzyme activities based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis, method optimisation considering kinetic studies to establish the optimal assay incubation time | Rubus idaeus | ? | - |
? | |
| 1.14.11.9 | additional information | development of a reverse phase (C18) HPLC method (with elutant ethylacetate) for the determination of flavanone 3beta-hydroxylase and other enzyme activities based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis, method optimisation considering kinetic studies to establish the optimal assay incubation time | Fragaria x ananassa | ? | - |
? | |
| 1.14.11.9 | additional information | development of a reverse phase (C18) HPLC method (with elutant ethylacetate) for the determination of flavanone 3beta-hydroxylase and other enzyme activities based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis, method optimisation considering kinetic studies to establish the optimal assay incubation time | Vaccinium myrtillus | ? | - |
? | |
| 1.14.11.9 | additional information | development of a reverse phase (C18) HPLC method (with elutant ethylacetate) for the determination of flavanone 3beta-hydroxylase and other enzyme activities based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis, method optimisation considering kinetic studies to establish the optimal assay incubation time | Ribes rubrum | ? | - |
? | |
| 1.14.11.9 | additional information | development of a reverse phase (C18) HPLC method (with elutant ethylacetate) for the determination of flavanone 3beta-hydroxylase and other enzyme activities based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis, method optimisation considering kinetic studies to establish the optimal assay incubation time | Ribes nigrum | ? | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Rubus idaeus | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Fragaria x ananassa | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Vaccinium myrtillus | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Ribes rubrum | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + 2-oxoglutarate + O2 | - |
Ribes nigrum | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + O2 + 2-oxoglutarate | - |
Rubus plicatus | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + O2 + 2-oxoglutarate | - |
Rubus idaeus | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + O2 + 2-oxoglutarate | - |
Ribes nigrum | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + O2 + 2-oxoglutarate | - |
Fragaria x ananassa | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | naringenin + O2 + 2-oxoglutarate | - |
Ribes rubrum | dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Rubus plicatus | kaempferol + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Rubus idaeus | kaempferol + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Ribes nigrum | kaempferol + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Fragaria x ananassa | kaempferol + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Ribes rubrum | kaempferol + succinate + CO2 + H2O | - |
? | |
| 4.3.1.24 | L-phenylalanine | - |
Rubus plicatus | (E)-cinnamate + NH3 | - |
? | |
| 4.3.1.24 | L-phenylalanine | - |
Fragaria x ananassa | (E)-cinnamate + NH3 | - |
? | |
| 4.3.1.24 | L-phenylalanine | - |
Ribes nigrum | (E)-cinnamate + NH3 | - |
? | |
| 4.3.1.24 | L-phenylalanine | - |
Ribes rubrum | (E)-cinnamate + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | F3H | - |
Vaccinium myrtillus |
| 1.14.11.9 | F3H | - |
Ribes rubrum |
| 1.14.11.9 | FHT | - |
Rubus plicatus |
| 1.14.11.9 | FHT | - |
Rubus idaeus |
| 1.14.11.9 | FHT | - |
Ribes nigrum |
| 1.14.11.9 | FHT | - |
Fragaria x ananassa |
| 1.14.11.9 | FHT | - |
Ribes rubrum |
| 1.14.11.9 | FHT | - |
Vaccinium myrtillus |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Rubus idaeus |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Fragaria x ananassa |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Vaccinium myrtillus |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Ribes rubrum |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Ribes nigrum |
| 1.14.20.6 | FLS | - |
Rubus plicatus |
| 1.14.20.6 | FLS | - |
Rubus idaeus |
| 1.14.20.6 | FLS | - |
Ribes nigrum |
| 1.14.20.6 | FLS | - |
Fragaria x ananassa |
| 1.14.20.6 | FLS | - |
Ribes rubrum |
| 4.3.1.24 | PAL | - |
Rubus plicatus |
| 4.3.1.24 | PAL | - |
Fragaria x ananassa |
| 4.3.1.24 | PAL | - |
Ribes nigrum |
| 4.3.1.24 | PAL | - |
Ribes rubrum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | 30 | - |
assay at | Rubus plicatus |
| 1.14.11.9 | 30 | - |
assay at | Rubus idaeus |
| 1.14.11.9 | 30 | - |
assay at | Ribes nigrum |
| 1.14.11.9 | 30 | - |
assay at | Fragaria x ananassa |
| 1.14.11.9 | 30 | - |
assay at | Ribes rubrum |
| 1.14.20.6 | 30 | - |
assay at | Rubus plicatus |
| 1.14.20.6 | 30 | - |
assay at | Rubus idaeus |
| 1.14.20.6 | 30 | - |
assay at | Ribes nigrum |
| 1.14.20.6 | 30 | - |
assay at | Fragaria x ananassa |
| 1.14.20.6 | 30 | - |
assay at | Ribes rubrum |
| 4.3.1.24 | 30 | - |
assay at | Rubus plicatus |
| 4.3.1.24 | 30 | - |
assay at | Fragaria x ananassa |
| 4.3.1.24 | 30 | - |
assay at | Ribes nigrum |
| 4.3.1.24 | 30 | - |
assay at | Ribes rubrum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | 7.3 | - |
assay at | Rubus plicatus |
| 1.14.11.9 | 7.3 | - |
assay at | Rubus idaeus |
| 1.14.11.9 | 7.3 | - |
assay at | Ribes nigrum |
| 1.14.11.9 | 7.3 | - |
assay at | Fragaria x ananassa |
| 1.14.11.9 | 7.3 | - |
assay at | Ribes rubrum |
| 1.14.20.6 | 7.3 | - |
assay at | Rubus plicatus |
| 1.14.20.6 | 7.3 | - |
assay at | Rubus idaeus |
| 1.14.20.6 | 7.3 | - |
assay at | Ribes nigrum |
| 1.14.20.6 | 7.3 | - |
assay at | Fragaria x ananassa |
| 1.14.20.6 | 7.3 | - |
assay at | Ribes rubrum |
| 4.3.1.24 | 7.3 | - |
assay at | Rubus plicatus |
| 4.3.1.24 | 7.3 | - |
assay at | Fragaria x ananassa |
| 4.3.1.24 | 7.3 | - |
assay at | Ribes nigrum |
| 4.3.1.24 | 7.3 | - |
assay at | Ribes rubrum |
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