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Literature summary extracted from

  • Ghatge, M.S.; Karve, S.S.; David, T.M.; Ahmed, M.H.; Musayev, F.N.; Cunningham, K.; Schirch, V.; Safo, M.K.
    Inactive mutants of human pyridoxine 5-phosphate oxidase a possible role for a noncatalytic pyridoxal 5-phosphate tight binding site (2016), FEBS Open Bio, 6, 398-408 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.5 R229W the mutation leads to deficiency of pyridoxal 5'-phosphate in the cell and causes neonatal epileptic encephalopathy Homo sapiens
1.4.3.5 R95C the mutation leads to deficiency of pyridoxal 5'-phosphate in the cell and causes neonatal epileptic encephalopathy. The mutant exhibits a 15fold reduction in affinity for the FMN cofactor, a 71fold decrease in affinity for the substrate pyridoxine 5'-phosphate, a 4.9fold decrease in specific activity, and a 343fold reduction in catalytic activity, compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.00618
-
 pyridoxine 5'-phosphate wild type enzyme, at pH 8.0 and 37°C Homo sapiens
1.4.3.5 0.436
-
 pyridoxine 5'-phosphate mutant enzyme R95C, at pH 8.0 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 Homo sapiens
-
 pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + O2 Homo sapiens
-
 pyridoxal 5'-phosphate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Homo sapiens Q9NVS9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Homo sapiens pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + O2
-
 Homo sapiens pyridoxal 5'-phosphate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 PNPO
-
 Homo sapiens
1.4.3.5 pyridoxine 5'-phosphate oxidase`
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.5 0.037
-
 pyridoxine 5'-phosphate mutant enzyme R95C, at pH 8.0 and 37°C Homo sapiens
1.4.3.5 0.18
-
 pyridoxine 5'-phosphate wild type enzyme, at pH 8.0 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.5 0.085
-
 pyridoxine 5'-phosphate mutant enzyme R95C, at pH 8.0 and 37°C Homo sapiens
1.4.3.5 29
-
 pyridoxine 5'-phosphate wild type enzyme, at pH 8.0 and 37°C Homo sapiens