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Literature summary extracted from

  • Watanabe, S.; Tanimoto, Y.; Yamauchi, S.; Tozawa, Y.; Sawayama, S.; Watanabe, Y.
    Identification and characterization of trans-3-hydroxy-l-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria (2014), FEBS Open Bio, 4, 240-250 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.1 gene lhpI, DNA and amino acid sequence determination and analysis, phylogenetic tree, genetic organization of genes encoding enzyme involved in the trans-3-hydroxy-L-proline pathway and networks with D-lysine and D-proline, overview. Recombinant expression of His6-tagged enzyme in Escherichia coli Azospirillum brasilense
1.5.1.21 gene lhpD, DNA and amino acid sequence determination and analysis, phylogenetic tree, genetic organization of genes encoding enzyme involved in the trans-3-hydroxy-L-proline pathway and networks with D-lysine and D-proline, overview. Recombinant expression of His6-tagged enzyme in Escherichia coli Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.1 additional information generation of a lphI knockout mutant, AbLhpI-, the mutant strain is distinct from the wild-type strain in that trans-3-hydroxy-L-proline, D-proline, and D-lysine do not support growth as a sole carbon source, but there is no difference in growth on other carbon sources, including trans-4-hydroxy-L-proline, between the two strains Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.1 0.474
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.474
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 0.491
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.491
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 0.6
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.6
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.1 0.628
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.628
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 0.837
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.837
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.1 2.1
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 2.79
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 3.63
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 3.99
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 5.9
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 6.54
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 7.26
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 7.26
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 14.8
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 18.3
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 18.3
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.1 18.8
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 18.8
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
1.5.1.1 80
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 80.1
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.21 0.447
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 0.835
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 1.57
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 18.5
-
 L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 34.8
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 132
-
 trans-3-hydroxy-L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.1 33312
-
 calculated from sequence Azospirillum brasilense
1.5.1.1 36182
-
 calculated from sequence Colwellia psychrerythraea
1.5.1.1 37000
-
 SDS-PAGE Azospirillum brasilense
1.5.1.1 40000
-
 SDS-PAGE Colwellia psychrerythraea
1.5.1.1 62000
-
 recombinant enzyme, gel filtration Azospirillum brasilense
1.5.1.1 78000
-
 gel filtration Azospirillum brasilense
1.5.1.1 81000
-
 gel filtration Colwellia psychrerythraea
1.5.1.21 78000
-
 recombinant enzyme, gel filtration Pseudomonas aeruginosa
4.2.1.77 37806
-
 calculated from sequence Azospirillum brasilense
4.2.1.77 40000
-
 SDS-PAGE Azospirillum brasilense
4.2.1.77 87000
-
 gel filtration Azospirillum brasilense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.1 1-piperideine-2-carboxylate + NAD(P)H + H+ Azospirillum brasilense
-
 L-pipecolate + NAD(P)+
-
 r
1.5.1.1 1-pyrroline-2-carboxylate + NAD(P)H + H+ Azospirillum brasilense
-
 L-proline + NAD(P)+
-
 r
1.5.1.1 additional information Azospirillum brasilense trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate ?
-
 ?
1.5.1.21 1-piperideine-2-carboxylate + NADPH + H+ Pseudomonas aeruginosa
-
 L-pipecolate + NADP+
-
 r
1.5.1.21 1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+ Pseudomonas aeruginosa
-
 (4R)-hydroxy-L-proline + NADP+
-
 r
1.5.1.21 1-pyrroline-2-carboxylate + NADPH + H+ Pseudomonas aeruginosa
-
 L-proline + NADP+
-
 r
1.5.1.21 cis-4-hydroxy-L-proline + NADPH + H+ Pseudomonas aeruginosa
-
 ? + NADP+
-
 r
4.2.1.77 trans-3-hydroxy-L-proline Azospirillum brasilense
-
 1-pyrroline 2-carboxylate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.1 Azospirillum brasilense V5YW53
-
-
1.5.1.1 Colwellia psychrerythraea Q485R8
-
-
1.5.1.21 Pseudomonas aeruginosa Q9I492
-
-
4.2.1.77 Azospirillum brasilense V5YXI5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.1 additional information the organsim can grow on trans-3-hydroxy-L-proline and trans-4-hydroxy-L-proline as sole carbom sources, but also on D-proline and D-lysine Azospirillum brasilense
-
1.5.1.21 additional information the organism cannot grow on trans-3-hydroxy-L-proline as sole carbon source Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.1 0.000457
-
 substrates: L-pipecolate, NAD+, pH 10.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.0005
-
 purified recombinant His-tagged enzyme, substrates L-pipecolate and NAD+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.00435
-
 substrates: L-pipecolate, NADP+, pH 10.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.0044
-
 purified recombinant His-tagged enzyme, substrates L-pipecolate and NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.0045
-
 purified recombinant His-tagged enzyme, substrates L-proline and NAD+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.00451
-
 substrates: L-proline, NAD+, pH 10.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.0464
-
 substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.174
-
 substrates: (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.291
-
 substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.1 0.63
-
 purified recombinant His-tagged enzyme, substrates L-proline and NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.63
-
 substrates: L-proline, NADP+, pH 10.5, 30°C Colwellia psychrerythraea
1.5.1.1 2.72
-
 substrates: L-pipecolate, NAD+, pH 10.5, 30°C Azospirillum brasilense
1.5.1.1 2.92
-
 substrates: L-pipecolate, NADP+, pH 10.5, 30°C Azospirillum brasilense
1.5.1.1 4.76
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.1 5.18
-
 substrates: L-proline, NADP+, pH 10.5, 30°C Azospirillum brasilense
1.5.1.1 5.5
-
 substrates: L-proline, NAD+, pH 10.5, 30°C Azospirillum brasilense
1.5.1.1 12
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-(4R)-hydroxy-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 12
-
 substrates: DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 30.2
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.1 177
-
 purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 177
-
 substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 220
-
 purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 220
-
 substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 584
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 584
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 600
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 600
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.21 0.49
-
 purified recombinant His-tagged enzyme, substrate trans-3-hydroxy-L-proline , pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 0.679
-
 purified recombinant His-tagged enzyme, substrate L-pipecolate, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 1.98
-
 purified recombinant His-tagged enzyme, substrate L-proline, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 12.1
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-(4R)-hydroxy-2-carboxylate and NADH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 42.3
-
 purified recombinant His-tagged enzyme, substrate 1-piperideine-2-carboxylate, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 42.3
-
 purified recombinant His-tagged enzyme, substrate 1-pyrroline-2-carboxylate, pH 7.0, 30°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.1 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea (4R)-4-hydroxy-L-proline + NADP+
-
 r
1.5.1.1 1-piperideine-2-carboxylate + NAD(P)H + H+
-
 Azospirillum brasilense L-pipecolate + NAD(P)+
-
 r
1.5.1.1 1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense (4R)-hydroxy-L-proline + NADP+
-
 r
1.5.1.1 1-pyrroline-2-carboxylate + NAD(P)H + H+
-
 Azospirillum brasilense L-proline + NAD(P)+
-
 r
1.5.1.1 cis-3-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r
1.5.1.1 cis-4-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r
1.5.1.1 DELTA1-piperidine-2-carboxylate + NADH + H+
-
 Azospirillum brasilense L-pipecolate + NAD+
-
 r
1.5.1.1 DELTA1-piperidine-2-carboxylate + NADH + H+
-
 Colwellia psychrerythraea L-pipecolate + NAD+
-
 r
1.5.1.1 DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense L-pipecolate + NADP+
-
 r
1.5.1.1 DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea L-pipecolate + NADP+
-
 r
1.5.1.1 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense (4R)-hydroxy-L-proline + NADP+
-
 r
1.5.1.1 DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 Azospirillum brasilense L-proline + NAD+
-
 r
1.5.1.1 DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 Colwellia psychrerythraea L-proline + NAD+
-
 r
1.5.1.1 DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense L-proline + NADP+
-
 r
1.5.1.1 DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea L-proline + NADP+
-
 r
1.5.1.1 L-pipecolate + NAD(P)+
-
 Azospirillum brasilense 1-piperideine-2-carboxylate + NAD(P)H + H+
-
 r
1.5.1.1 L-pipecolate + NAD+
-
 Azospirillum brasilense DELTA1-piperidine-2-carboxylate + NADH + H+
-
 r
1.5.1.1 L-pipecolate + NAD+
-
 Colwellia psychrerythraea DELTA1-piperidine-2-carboxylate + NADH + H+
-
 r
1.5.1.1 L-pipecolate + NADP+
-
 Azospirillum brasilense DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 r
1.5.1.1 L-pipecolate + NADP+
-
 Colwellia psychrerythraea DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 r
1.5.1.1 L-proline + NAD(P)+
-
 Azospirillum brasilense 1-pyrroline-2-carboxylate + NAD(P)H + H+
-
 r
1.5.1.1 L-proline + NAD+
-
 Azospirillum brasilense DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
1.5.1.1 L-proline + NAD+
-
 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
1.5.1.1 L-proline + NADP+
-
 Azospirillum brasilense DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
1.5.1.1 L-proline + NADP+
-
 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
1.5.1.1 additional information trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate Azospirillum brasilense ?
-
 ?
1.5.1.1 additional information no activity with trans-4-hydroxy-D-proline, D-proline, trans-3-hydroxy-L-proline, cis-4-hydroxy-D-proline, and cis-3-hydroxy-D-proline, poor activity with trans-4-hydroxy-L-proline Azospirillum brasilense ?
-
 ?
1.5.1.1 trans-4-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r
1.5.1.21 1-piperideine-2-carboxylate + NADPH + H+
-
 Pseudomonas aeruginosa L-pipecolate + NADP+
-
 r
1.5.1.21 1-piperideine-2-carboxylate + NADPH + H+ the reaction equilibrium favors the direction toward NADPH-dependent reduction Pseudomonas aeruginosa L-pipecolate + NADP+
-
 r
1.5.1.21 1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+
-
 Pseudomonas aeruginosa (4R)-hydroxy-L-proline + NADP+
-
 r
1.5.1.21 1-pyrroline-2-carboxylate + NADPH + H+
-
 Pseudomonas aeruginosa L-proline + NADP+
-
 r
1.5.1.21 1-pyrroline-2-carboxylate + NADPH + H+ the reaction equilibrium favors the direction toward NADPH-dependent reduction Pseudomonas aeruginosa L-proline + NADP+
-
 r
1.5.1.21 cis-3-hydroxy-L-proline + NADPH + H+ low activity Pseudomonas aeruginosa ? + NADP+
-
 r
1.5.1.21 cis-4-hydroxy-L-proline + NADPH + H+
-
 Pseudomonas aeruginosa ? + NADP+
-
 r
1.5.1.21 cis-4-hydroxy-L-proline + NADPH + H+ low activity Pseudomonas aeruginosa ? + NADP+
-
 r
1.5.1.21 L-pipecolate + NADP+ the reaction equilibrium favors the direction toward NADPH-dependent reduction Pseudomonas aeruginosa 1-piperideine-2-carboxylate + NADPH + H+
-
 r
1.5.1.21 L-proline + NADP+ the reaction equilibrium favors the direction toward NADPH-dependent reduction Pseudomonas aeruginosa 1-pyrroline-2-carboxylate + NADPH + H+
-
 r
1.5.1.21 additional information PaLhpD shows 32.8%, 14.4% and 12.2% activity for trans-3-hydroxy-L-proline, cis-4-hydroxy-L-proline, and cis-3-hydroxy-L-proline as a percent of L-proline in the oxidation reaction, respectively. Both L-proline and L-pipecolate undergo NADP+-dependent oxidization, but their kcat/Km values are 500 and 350fold lower than those with 1-pyrroline-2-carboxylate and 1-piperideine-2-carboxylate, respectively. No activity with trans-4-hydroxy-D-proline, D-proline, cis-4-hydroxy-D-proline, and cis-3-hydroxy-D-proline, poor activity with trans-4-hydroxy-L-proline Pseudomonas aeruginosa ?
-
 ?
1.5.1.21 trans-3-hydroxy-L-proline + NADPH + H+ moderate activity Pseudomonas aeruginosa ? + NADP+
-
 r
4.2.1.77 trans-3-hydroxy-L-proline
-
 Azospirillum brasilense 1-pyrroline 2-carboxylate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.1 dimer 2 * 40000, SDS-PAGE Colwellia psychrerythraea
1.5.1.1 dimer 2 * 37000, SDS-PAGE Azospirillum brasilense
1.5.1.1 dimer 2 * 33312, calculated from sequence Azospirillum brasilense
1.5.1.1 dimer 2 * 36182, calculated from sequence Colwellia psychrerythraea
1.5.1.1 homodimer 2 * 33313, sequence calculation, 2 * 37000, recombinant enzyme, SDS-PAGE Azospirillum brasilense
1.5.1.21 homodimer 2 * 37165, sequence calculation, 2 * 40000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
4.2.1.77 dimer 2 * 40000, SDS-PAGE Azospirillum brasilense
4.2.1.77 dimer 2 * 37806, calculated from sequence Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
1.5.1.1 CphpI
-
 Colwellia psychrerythraea
1.5.1.1 DELTA1-Pyr2C reductase
-
 Azospirillum brasilense
1.5.1.1 DELTA1-Pyr2C reductase
-
 Colwellia psychrerythraea
1.5.1.1 DELTA1-pyrroline-2-carboxylate reductase
-
 Azospirillum brasilense
1.5.1.1 DELTA1-pyrroline-2-carboxylate reductase
-
 Colwellia psychrerythraea
1.5.1.1 lhpI
-
 Azospirillum brasilense
1.5.1.1 NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase
-
 Azospirillum brasilense
1.5.1.1 Pyr2C reductase
-
 Azospirillum brasilense
1.5.1.1 Pyr2C/Pip2C reductase
-
 Azospirillum brasilense
1.5.1.21 DELTA1-pyrroline-2-carboxylate reductase
-
 Pseudomonas aeruginosa
1.5.1.21 lhpD
-
 Pseudomonas aeruginosa
1.5.1.21 NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase
-
 Pseudomonas aeruginosa
1.5.1.21 PaLhpD
-
 Pseudomonas aeruginosa
1.5.1.21 Pyr2C reductase
-
 Pseudomonas aeruginosa
1.5.1.21 Pyr2C/Pip2C reductase
-
 Pseudomonas aeruginosa
4.2.1.77 LhpH
-
 Azospirillum brasilense
4.2.1.77 T3LHyp dehydratase
-
 Azospirillum brasilense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.1 30
-
 assay at Azospirillum brasilense
1.5.1.21 30
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.1 0.0069
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.0069
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
1.5.1.1 0.02
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.1 0.0213
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.088
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 1.02
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 1.09
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 1.09
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.1 1.38
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 2.5
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 3.7
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 3.92
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 4.23
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 10.35
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 10.35
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 10.35
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 124.5
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 161.5
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 161.5
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.1 165.8
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 166
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 523
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 523.3
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 615
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 615
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.21 2.25
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 3.42
-
 L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 4.53
-
 trans-3-hydroxy-L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 14.5
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 35.3
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 41.67
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.1 6.5
-
 reduction reaction Azospirillum brasilense
1.5.1.1 10.5
-
 oxidation reaction Azospirillum brasilense
1.5.1.21 7
-
 reduction reaction of 1-pyrroline-2-carboxylate and 1-piperideine-2-carboxylate Pseudomonas aeruginosa
1.5.1.21 10
-
 oxidation reaction of L-proline and L-pipecolate Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.1 additional information the enzyme shows dual specificity for NADPH and NADH Azospirillum brasilense
1.5.1.1 NADH
-
 Azospirillum brasilense
1.5.1.1 NADPH
-
 Azospirillum brasilense
1.5.1.21 additional information no activity with NADH Pseudomonas aeruginosa
1.5.1.21 NADP+
-
 Pseudomonas aeruginosa
1.5.1.21 NADPH
-
 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
1.5.1.1 Azospirillum brasilense trans-3-hydroxy-L-proline dehydratase and NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase activity is induced by trans-3-hydroxy-L-proline, D-proline, and D-lysine, but not by trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline up
1.5.1.1 Azospirillum brasilense induced by trans-3-hydroxy-L-proline (and D-proline and D-lysine) but not trans-4-hydroxy-L-proline up
4.2.1.77 Azospirillum brasilense induced by trans-3-hydroxy-L-proline (and D-proline and D-lysine) but not trans-4-hydroxy-L-proline up

General Information

EC Number General Information Comment Organism
1.5.1.1 evolution enzyme Pyr2C reductase is a member of the ornithine cyclodeaminase/micro-crystallin superfamily, different from known dpkA protein, and there are several significant differences in the enzymatic properties between Azospirillum brasilense and another bacteria: substrate and coenzyme specificities, phylogenetic analysis Azospirillum brasilense
1.5.1.1 malfunction disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline (T3LHyp), D-proline and D-lysine Azospirillum brasilense
1.5.1.1 malfunction disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline, D-proline and D-lysine, indicating that this gene has dual metabolic functions as a reductase for Pyr2C and DELTA1-piperidine-2-carboxylate in these pathways, and that the T3LHyp pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism Azospirillum brasilense
1.5.1.1 metabolism the enzyme is involved in the trans-3-hydroxy-L-proline pathway and the metabolic networks with D-lysine and D-proline, overview. The trans-3-hydroxy-L-proline pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism Azospirillum brasilense
1.5.1.1 physiological function enzyme AbLhpI is a bifunctional NAD(P)H-dependent Delta1-pyrroline-2-carboxylate/Delta1-piperideine-2-carboxylate reductase (Pyr2C/Pip2C reductase) Azospirillum brasilense
1.5.1.21 evolution enzyme Pyr2C reductase is a member of the ornithine cyclodeaminase/micro-crystallin superfamily and behaves similar to DkpA Pseudomonas aeruginosa
1.5.1.21 metabolism the enzyme is not involved in the trans-3-hydroxy-L-proline pathway and the trans-4-hydroxy-L-proline pathway Pseudomonas aeruginosa
4.2.1.77 metabolism the enzyme is involved in the trans-3-hydroxy-L-proline pathway. The trans-3-hydroxy-L-proline pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism Azospirillum brasilense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.1 0.000027
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.1 0.00027
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.00037
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.00037
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
1.5.1.1 0.0418
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 0.0595
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.5.1.1 0.06
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 0.124
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 0.19
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 0.25
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 0.38
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 1.06
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.5.1.1 1.08
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.5.1.1 21.1
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 21.2
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.5.1.1 21.2
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 225
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.5.1.1 269
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 270
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.1 350
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 351.7
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.1 735
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 735
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
1.5.1.1 833
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
1.5.1.1 835
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
1.5.1.21 0.034
-
 trans-3-hydroxy-L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 0.065
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 0.185
-
 L-proline purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 17.3
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 22.5
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa
1.5.1.21 93.2
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 7.0, 30°C Pseudomonas aeruginosa