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Literature summary extracted from
- The X-ray crystal structure of APR-B, an atypical adenosine 5-phosphosulfate reductase from Physcomitrella patens (2013), FEBS Lett., 587, 3626-3632 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.4.10 | crystal structure is consistent with a preference for adenosine 5'-phosphosulfate. The [Fe4-S4] cluster enhances the enzyme's activity | Physcomitrium patens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.10 | D122G | mutant does not display improved activity with 3'-phosphoadenosine-5'-phosphosulfate and shows significantly reduced activity with 5'-adenylylsulfate as the substrate | Physcomitrium patens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.10 | 0.0058 | - |
5'-adenylyl sulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens |  |
| 1.8.4.10 | 0.0069 | - |
5'-adenylyl sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.04 | - |
3'-phosphoadenosine-5'-phosphosulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.049 | - |
3'-phosphoadenosine-5'-phosphosulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.10 | 64000 | - |
gel filtration | Physcomitrium patens |
| 1.8.4.10 | 71000 | - |
dynamic light scattering | Physcomitrium patens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.10 | Physcomitrium patens | Q8L5D0 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.10 | 3'-phosphoadenosine-5'-phosphosulfate + thioredoxin | substrate of phosphoadenylyl-sulfate reductase, EC 1.8.4.8 | Physcomitrium patens | ? + thioredoxin disulfide | - |
? | |
| 1.8.4.10 | 5'-adenylyl sulfate + thioredoxin | - |
Physcomitrium patens | AMP + sulfite + thioredoxin disulfide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.10 | dimer | crystallization data | Physcomitrium patens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.10 | 0.0001 | - |
3'-phosphoadenosine-5'-phosphosulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.0002 | - |
3'-phosphoadenosine-5'-phosphosulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.015 | - |
5'-adenylyl sulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.333 | - |
5'-adenylyl sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.10 | [4Fe-4S]-center | - |
Physcomitrium patens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.10 | 0.0034 | - |
3'-phosphoadenosine-5'-phosphosulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 0.0037 | - |
3'-phosphoadenosine-5'-phosphosulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 2.6 | - |
5'-adenylyl sulfate | mutant D122G, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
| 1.8.4.10 | 48.3 | - |
5'-adenylyl sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Physcomitrium patens | |
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