EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.7 | expression in Escherichia coli | Colwellia psychrerythraea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.7 | 0.0045 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
1.8.1.7 | 0.0109 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.7 | 95240 | - |
gel filtration | Colwellia psychrerythraea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.7 | Colwellia psychrerythraea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.7 | color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins | Colwellia psychrerythraea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.7 | glutathione disulfide + NADPH + H+ | - |
Colwellia psychrerythraea | glutathione + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.7 | dimer | 2 * 50000, SDS-PAGE, 2 * 48700, MALDI-TOF | Colwellia psychrerythraea |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.7 | 45 | - |
3 h, 75% resiudal activity | Colwellia psychrerythraea |
1.8.1.7 | 50 | - |
half-life 40 min | Colwellia psychrerythraea |
1.8.1.7 | 55 | - |
half-life 8 min | Colwellia psychrerythraea |
1.8.1.7 | 60 | - |
half-life below 2 min | Colwellia psychrerythraea |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.7 | 217 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
1.8.1.7 | 700 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.7 | FAD | color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins | Colwellia psychrerythraea | |
1.8.1.7 | NADPH | - |
Colwellia psychrerythraea |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.1.7 | physiological function | gene is able to complement an Escherichia coli glutathione reductase deletion strain in oxidative stress growth assays | Colwellia psychrerythraea |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.7 | 46.7 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
1.8.1.7 | 63.3 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |