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Literature summary extracted from

  • Haase, P.; Deppenmeier, U.; Blaut, M.; Gottschalk, G.
    Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius (1992), Eur. J. Biochem., 203, 527-531 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.98.3 the purified enzyme becomes rapidly inactivated by repeated freezing and thawing Methanolobus tindarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.3 0.0054
-
 reduced coenzyme F420 at pH 6.8 and 37°C Methanolobus tindarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.98.3 membrane
-
 Methanolobus tindarius 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.98.3 Iron 16 mol non-heme iron per mol enzyme Methanolobus tindarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.3 120000
-
 gel filtration Methanolobus tindarius

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.3 Methanolobus tindarius
-
-
-
1.5.98.3 Methanolobus tindarius DSM 2278
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.3 DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography Methanolobus tindarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.3 reduced coenzyme F420 + oxidized metronidazole
-
 Methanolobus tindarius oxidized coenzyme F420 + reduced metronidazole
-
 ?
1.5.98.3 reduced coenzyme F420 + oxidized metronidazole
-
 Methanolobus tindarius DSM 2278 oxidized coenzyme F420 + reduced metronidazole
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.98.3 ? x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE Methanolobus tindarius

Synonyms

EC Number Synonyms Comment Organism
1.5.98.3 F420H2-dehydrogenase
-
 Methanolobus tindarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.3 37
-
-
 Methanolobus tindarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.98.3 25.5
-
 reduced coenzyme F420 at pH 6.8 and 37°C Methanolobus tindarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.3 6.8
-
-
 Methanolobus tindarius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.98.3 Methanolobus tindarius isoelectric focusing
-
 5