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Literature summary extracted from

  • Monteoliva, M.I.; Rizzi, Y.S.; Cecchini, N.M.; Hajirezaei, M.R.; Alvarez, M.E.
    Context of action of proline dehydrogenase (ProDH) in the hypersensitive response of Arabidopsis (2014), BMC Plant Biol., 14, 21 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 semi-quantitative, and quantitative RT-PCR enzyme expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.5.99.B2 additional information mutant plants lacking DELTA1 pyrroline-5-carboxylate dehydrogenase activate ProDH during hypersensitive response like the wild-type plants. They achieve maximum oxidative burst and cell death levels producing normal hypersensitive response lesions, but evidence premature defense activation Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.B2 mitochondrial inner membrane
-
 Arabidopsis thaliana 5743
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.B2 L-proline + acceptor Arabidopsis thaliana
-
 (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.99.B2 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + acceptor
-
 Arabidopsis thaliana (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 Pro dehydrogenase
-
 Arabidopsis thaliana
1.5.99.B2 PRODH
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD ProDH is a flavoenzyme, that can eventually transfer electrons to O2 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.5.99.B2 Arabidopsis thaliana oxidative burst causes a hypersensitive response with induction of proline dehydrogenase (ProDH), the plants do not consume Pro during maximal reactive oxygen species accumulation, and maintain almost basal DELTA1 pyrroline-5-carboxylate levels at all conditions. Mutant plants lacking DELTA1 pyrroline-5-carboxylate dehydrogenase activate ProDH like the wild-type plants. Under cold acclimation, ProDH induction is accompanied by Pro increase. ProDH activation occuring after stress release does not always be accompanied by P5CDH and can have minor effects on the Pro content up

General Information

EC Number General Information Comment Organism
1.5.99.B2 physiological function proline (Pro) dehydrogenase (ProDH) catalyzes the rate limiting step in the oxidation pathway, transferring two electrons from Pro to the noncovalently bound FAD cofactor to subsequently deliver these electrons to a final acceptor. The plant enzyme sustains oxidative phosphorylation and ATP generation using endogenous electron acceptors. ProDH potentiates the oxidative burst and cell death of the plant hypersensitive response. ProDH converts Pro into DELTA1 pyrroline-5-carboxylate (P5C) and can act together with P5C dehydrogenase (P5CDH) to produce Glu, or with P5C reductase (P5CR) to regenerate Pro and thus stimulate the Pro/P5C cycle. ProDH activation has different effects on hypersensitive response. Before the oxidative burst it leads to Pro consumption involving the action of P5CDH. During the oxidative burst, ProDH becomes functionally uncoupled to P5CDH and apparently works with P5C reductase. The absence of P5CDH does not reduce reactive oxygen species, cell death, or pathogen resistance, indicating this enzyme is not accompanying ProDH in the potentiation of these defense responses. In contrast, p5cdh infected mutant plants display increased reactive oxygen species burst and earlier initiation of hypersensitive response cell death. ProDH may sustain hypersensitive response by participating in the Pro/P5C cycle. Evaluation of ProDH action at different hypersensitive response stages, overview Arabidopsis thaliana