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Literature summary extracted from

  • Dua, A.; Joshi, S.; Satyanarayana, T.
    Recombinant exochitinase of the thermophilic mould Myceliophthora thermophila BJA Characteristics and utility in generating N-acetyl glucosamine and in biocontrol of phytopathogenic fungi (2017), Biotechnol. Prog., 33, 70-80 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 5 mM, 7% residual activity Thermothelomyces thermophilus
3.2.1.14 Ag+ 5 mM, 66% residual activity Thermothelomyces thermophilus
3.2.1.14 Cd2+ 5 mM, 69% residual activity Thermothelomyces thermophilus
3.2.1.14 Hg2+ 5 mM, 60% residual activity Thermothelomyces thermophilus
3.2.1.14 iodoacetamide 5 mM, 51% residual activity Thermothelomyces thermophilus
3.2.1.14 Ni2+ 5 mM, 72% residual activity Thermothelomyces thermophilus
3.2.1.14 Tween-80 10%, 49% residual activity Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 colloidal chitin Km value 4.6 mg/ml, pH 5, 55°C Thermothelomyces thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.14 Acetone 10%, 83% residual activity Thermothelomyces thermophilus
3.2.1.14 benzene 10%, 70% residual activity Thermothelomyces thermophilus
3.2.1.14 Ethanol 10%, 81% residual activity Thermothelomyces thermophilus
3.2.1.14 Glycerol 10%, 84% residual activity Thermothelomyces thermophilus
3.2.1.14 hexane 10%, 70% residual activity Thermothelomyces thermophilus
3.2.1.14 isobutanol 10%, 83% residual activity Thermothelomyces thermophilus
3.2.1.14 toluene 10%, 76% residual activity Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Thermothelomyces thermophilus
-
-
-
3.2.1.14 Thermothelomyces thermophilus BJA
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Thermothelomyces thermophilus N-acetyl-D-glucosamine + ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Thermothelomyces thermophilus BJA N-acetyl-D-glucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 48000, SDS-PAGE Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chit
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 65
-
 half-life 113 min Thermothelomyces thermophilus
3.2.1.14 75
-
 half-life 48 min Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Thermothelomyces thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4
-
 3 h, 84% residual activity Thermothelomyces thermophilus

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function enzyme inhibits the growth of phytopathogenic fungi like Fusarium oxysporum and Curvularia lunata Thermothelomyces thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 colloidal chitin Km value 4.6 mg/ml, pH 5, 55°C Thermothelomyces thermophilus