Literature summary extracted from

Geng, F.; Ma, C.W.; Zeng, A.P.
Reengineering substrate specificity of E. coli glutamate dehydrogenase using a position-based prediction method (2017), Biotechnol. Lett., 39, 599-605 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.4.1.4	-	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.4.1.4	K92C	altering substrate specificity from glutamate to homoserine for a de novo 1,3-propanediol biosynthetic pathway, 5.5fold increase in speific activity with homoserine	Escherichia coli
1.4.1.4	K92M	altering substrate specificity from glutamate to homoserine for a de novo 1,3-propanediol biosynthetic pathway, 2.8fold increase in speific activity with homoserine	Escherichia coli
1.4.1.4	K92V	altering substrate specificity from glutamate to homoserine for a de novo 1,3-propanediol biosynthetic pathway, 7.2fold increase in speific activity with homoserine	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.4	Escherichia coli	Q8XDW9	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.4	L-glutamate + H2O + NADP+	-	Escherichia coli	2-oxoglutarate + NH3 + NADPH + H+	-	?
1.4.1.4	L-homoserine + H2O + NAD+	-	Escherichia coli	4-hydroxy-2-oxobutanoate + NH3 + NADH	-	?

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Release 2023.1 (January 2023)