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Literature summary extracted from

  • Fu, J.; Huo, G.; Feng, L.; Mao, Y.; Wang, Z.; Ma, H.; Chen, T.; Zhao, X.
    Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production (2016), Biotechnol. Biofuels, 9, 90 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene budC, phylogenetic tree, functional recombinant expression in Bacillus subtilis strain 168 Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B20 additional information Bacillus subtilis is engineered to produce chiral pure meso-2,3-BD. D-2,3-butanediol production is abolished by deleting D-2,3-butanediol dehydrogenase (EC 1.1.1.4) coding gene bdhA, and acoA gene is knocked out to prevent the degradation of acetoin, the immediate precursor of 2,3-butanediol. Next, both pta and ldh gene are deleted to decrease the accumulation of the byproducts, acetate and L-lactate. The meso-2,3-butanediol dehydrogenase coding gene from Klebsiella pneumoniae CICC10011 is introduced, as well as alsSD overexpressed in the tetra mutant (DELTAacoADELTAbdhADELTAptaDELTAldh) to achieve the efficient production of chiral meso-2,3-butanediol. Finally, the pool of NADH availability is further increased to facilitate the conversion of meso-2,3-butanediol from acetoin by overexpressing the udhA gene (coding a soluble transhydrogenase) and low dissolved oxygen control during the cultivation. Under microaerobic oxygen conditions, the best strain BSF9 produced 103.7 g/L meso-2,3-butanediol with a yield of 0.487 g/g glucose in the 5-L batch fermenter, and the titer of the main byproduct acetoin is no more than 1.1 g/L. Method optimization. The titer of meso-2,3-butanediol is almost unchanged at 37°C, 42°C, and 46°C, while the meso-2,3-butanediol productivity increases when the cultivation temperature is increased from 37°C to 46°C. The titer and productivity at 50°C decreases by 28.6% and 36.3% compared to those at 37°C Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+ Klebsiella pneumoniae
-
 acetoin + NADH + H+
-
 r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+ Klebsiella pneumoniae CICC10011
-
 acetoin + NADH + H+
-
 r
1.1.1.B20 acetoin + NADH + H+ Klebsiella pneumoniae
-
 (2R,3S)-butane-2,3-diol + NAD+
-
 r
1.1.1.B20 acetoin + NADH + H+ Klebsiella pneumoniae CICC10011
-
 (2R,3S)-butane-2,3-diol + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Klebsiella pneumoniae
-
-
-
1.1.1.B20 Klebsiella pneumoniae CICC10011
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae acetoin + NADH + H+
-
 r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae CICC10011 acetoin + NADH + H+
-
 r
1.1.1.B20 acetoin + NADH + H+
-
 Klebsiella pneumoniae (2R,3S)-butane-2,3-diol + NAD+
-
 r
1.1.1.B20 acetoin + NADH + H+
-
 Klebsiella pneumoniae CICC10011 (2R,3S)-butane-2,3-diol + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 budC
-
 Klebsiella pneumoniae
1.1.1.B20 meso-2,3-BD dehydrogenase
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
 Klebsiella pneumoniae
1.1.1.B20 NADH
-
 Klebsiella pneumoniae