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Literature summary extracted from

  • Fang, Y.; Bullock, H.; Lee, S.A.; Sekar, N.; Eiteman, M.A.; Whitman, W.B.; Ramasamy, R.P.
    Detection of methyl salicylate using bi-enzyme electrochemical sensor consisting salicylate hydroxylase and tyrosinase (2016), Biosens. Bioelectron., 85, 603-610 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.1 expressed in Escherichia coli XL1-Blue cells Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 HisTrap column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 additional information
-
 after 19fold purification, the enzyme shows a specific activity of 12.34 units/mg Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 NahG
-
 Pseudomonas putida
1.14.13.1 salicylate hydroxylase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD
-
 Pseudomonas putida
1.14.13.1 NADH
-
 Pseudomonas putida