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Literature summary extracted from
- Characterization of a novel thiosulfate dehydrogenase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH (2016), Biosci. Biotechnol. Biochem., 80, 273-278 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | additional information | the Acidithiobacillus thiooxidans strain SH enzyme is isolated to develop a bioleaching process for NaCl-containing sulfide minerals. Thiosulfate-metabolizing activity is most important for bioleaching, as the sulfur moiety of sulfide minerals is thought to be metabolized via thiosulfate as an intermediate | Acidithiobacillus thiooxidans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | 2-heptyl-4-hydroxy-quinoline N-oxide | strong inhibition | Acidithiobacillus thiooxidans |  |
| 1.8.99.B2 | 2-heptyl-4-hydroxyquinoline N-oxide | 88% inhibition at 0.001 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | 2-heptyl-4-hydroxyquinoline N-oxide | strong inhibition | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | N-ethylmaleimide | 16% inhibition at 1 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | Na+ | inhibitory at high concentration above 200 mM; inihibits at high concentrations | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | Na2SO3 | 70% inhibition at 1 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | Na2SO4 | 54% inhibition at 200 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | sulfite | - |
Acidithiobacillus thiooxidans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.99.B2 | membrane | - |
Acidithiobacillus thiooxidans | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | Cl- | activates | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | KCl | moderately stimulating, at 200 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | Mg2+ | activates | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | MgCl2 | moderately stimulating at 200 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | MgSO4 | slightly stimulating, at 200 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | additional information | no stimulation of the enzyme by Na2SO4 at at 100 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | NaCl | activates, best at 200 mM | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | NaCl | stimulates, best at 200 mM | Acidithiobacillus thiooxidans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 99000 | - |
gel filtration | Acidithiobacillus thiooxidans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.B2 | 2 thiosulfate + ubiquinone | Acidithiobacillus thiooxidans | - |
tetrathionate + ubiquinol | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ubiquinone | Acidithiobacillus thiooxidans SH | - |
tetrathionate + ubiquinol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.B2 | Acidithiobacillus thiooxidans | - |
- |
- |
| 1.8.99.B2 | Acidithiobacillus thiooxidans SH | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.B2 | native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration | Acidithiobacillus thiooxidans |
| 1.8.99.B2 | native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration | Acidithiobacillus thiooxidans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.99.B2 | culture condition:sulfur-grown cell | NaCl at an optimum concentration of 0.35 M stimulates the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth of strain SH | Acidithiobacillus thiooxidans | - |
| 1.8.99.B2 | additional information | NaCl at an optimum concentration of 0.35 M stimulated the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth | Acidithiobacillus thiooxidans | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 45 | - |
purified native enzyme, pH 4.0, 40°C | Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.B2 | 2 thiosulfate + ferricyanide | - |
Acidithiobacillus thiooxidans | tetrathionate + ferrocyanide | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ferricyanide | artificial electron acceptor | Acidithiobacillus thiooxidans | tetrathionate + ferrocyanide | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ferricyanide | - |
Acidithiobacillus thiooxidans SH | tetrathionate + ferrocyanide | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ferricyanide | artificial electron acceptor | Acidithiobacillus thiooxidans SH | tetrathionate + ferrocyanide | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ubiquinone | - |
Acidithiobacillus thiooxidans | tetrathionate + ubiquinol | - |
? | |
| 1.8.99.B2 | 2 thiosulfate + ubiquinone | - |
Acidithiobacillus thiooxidans SH | tetrathionate + ubiquinol | - |
? | |
| 1.8.99.B2 | additional information | no activity with cytochrome c | Acidithiobacillus thiooxidans | ? | - |
? | |
| 1.8.99.B2 | additional information | tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors | Acidithiobacillus thiooxidans | ? | - |
? | |
| 1.8.99.B2 | additional information | no activity with cytochrome c | Acidithiobacillus thiooxidans SH | ? | - |
? | |
| 1.8.99.B2 | additional information | tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors | Acidithiobacillus thiooxidans SH | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | homodimer | 2 * 44000, SDS-PAGE | Acidithiobacillus thiooxidans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | tetrathionate-forming TSD | - |
Acidithiobacillus thiooxidans |
| 1.8.99.B2 | thiosulfate dehydrogenase | - |
Acidithiobacillus thiooxidans |
| 1.8.99.B2 | thiosulfate:quinone reductase | - |
Acidithiobacillus thiooxidans |
| 1.8.99.B2 | TQO | - |
Acidithiobacillus thiooxidans |
| 1.8.99.B2 | TSD | - |
Acidithiobacillus thiooxidans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 40 | - |
- |
Acidithiobacillus thiooxidans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 100 | - |
inactivation | Acidithiobacillus thiooxidans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 4 | - |
- |
Acidithiobacillus thiooxidans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 2.5 | 5.5 | activity range, profile overview | Acidithiobacillus thiooxidans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | ferricyanide | - |
Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | additional information | no activity with cytochrome c from horse heart | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | additional information | the enzyme contains no c-type heme molecule | Acidithiobacillus thiooxidans | |
| 1.8.99.B2 | ubiquinone | - |
Acidithiobacillus thiooxidans | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | evolution | strain SH might have acquired a unique gene encoding the 44 kDa protein by horizontal transfer, as the protein with a high score and coverage is not found in other Acidithiobacillus thiooxidans genomes | Acidithiobacillus thiooxidans |
| 1.8.99.B2 | physiological function | a thiosulfate metabolizing enzyme is required for the sulfur-grown cells to metabolize the resulting thiosulfate | Acidithiobacillus thiooxidans |
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