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Literature summary extracted from

  • Takeda, K.; Ishida, T.; Igarashi, K.; Samejima, M.; Nakamura, N.; Ohno, H.
    Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase (2014), Biosci. Biotechnol. Biochem., 78, 1195-1198 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.2.8 Butylamine
-
Pseudomonas putida
1.1.2.8 Dimethylamine
-
Pseudomonas putida
1.1.2.8 dipropylamine
-
Pseudomonas putida
1.1.2.8 ethylamine inhibitory at high concentrations Pseudomonas putida
1.1.2.8 heptylamine
-
Pseudomonas putida
1.1.2.8 Hexylamine
-
Pseudomonas putida
1.1.2.8 Isobutylamine slight activation Pseudomonas putida
1.1.2.8 methylamine
-
Pseudomonas putida
1.1.2.8 additional information the enzyme requires ammonia or primary amines as activators in in vitro assays with artificial electron acceptors, the enzyme is activated by various primary amines, dimethylamine, and trimethylamine. tert-Butylamine is a poor activator. The hydrophobic interaction contributes to the binding of amine activators to the binding site in the enzyme Pseudomonas putida
1.1.2.8 octylamine
-
Pseudomonas putida
1.1.2.8 pentylamine the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations Pseudomonas putida
1.1.2.8 Propylamine
-
Pseudomonas putida
1.1.2.8 triethylamine
-
Pseudomonas putida
1.1.2.8 trimethylamine
-
Pseudomonas putida
1.1.2.8 tripropylamine
-
Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.8 ethylamine activating at low concentrations, inhibitory at high concentrations Pseudomonas putida
1.1.2.8 pentylamine the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.8 0.14
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
1.1.2.8 0.21
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
1.1.2.8 0.35
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
1.1.2.8 0.43
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.8 periplasm
-
Pseudomonas putida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.8 Ca2+ required Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.8 Pseudomonas putida
-
-
-
1.1.2.8 Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.8 ethanol + 2 cytochrome c
-
Pseudomonas putida ethanal + 2 reduced cytochrome c
-
?
1.1.2.8 ethanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 ethanal + 2 reduced cytochrome c
-
?
1.1.2.8 additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida ?
-
?
1.1.2.8 additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida KT 2240 ?
-
?
1.1.2.8 propanol + 2 cytochrome c
-
Pseudomonas putida propanal + 2 reduced cytochrome c
-
?
1.1.2.8 propanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 propanal + 2 reduced cytochrome c
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.8 PpADH
-
Pseudomonas putida
1.1.2.8 PQQ-ADH
-
Pseudomonas putida
1.1.2.8 pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.8 25
-
assay at Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.8 24.8
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
1.1.2.8 32.6
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
1.1.2.8 56.6
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
1.1.2.8 73.1
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.8 9
-
assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.8 cytochrome c natural electron acceptor Pseudomonas putida
1.1.2.8 additional information enzyme assays are performed with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida
1.1.2.8 pyrroloquinoline quinone prosthetic group, dependent on Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.2.8 37.6
-
pentylamine pH 9.0, temperature not specified in the publication Pseudomonas putida
1.1.2.8 112.5
-
ethylamine pH 9.0, temperature not specified in the publication Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.1.2.8 evolution ADHs are categorized into three groups (type I, II, and III ADHs) according to their domain structure and localization. Type I ADHs have molecular and enzymatic properties that are very similar to those of methanol dehydrogenases, MDHs, but they have a low affinity for methanol. Type I ADHs, on the other hand, generally use ethylamine or methylamine as essential activators instead of ammonia Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.2.8 73
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
1.1.2.8 115
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
1.1.2.8 209
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
1.1.2.8 395
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida