Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rezaei, S.; Shahverdi, A.R.; Faramarzi, M.A.
    Isolation, one-step affinity purification, and characterization of a polyextremotolerant laccase from the halophilic bacterium Aquisalibacillus elongatus and its application in the delignification of sugar beet pulp (2017), Biores. Technol., 230, 67-75 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 industry delignification of sugar beet pulp. Delignification pretreatment is the most challenging and critical step in the recycling process of lignocellulosic materials as the most abundant natural resources on the earth and has a large impact on cellulose extraction, cellulose digestibility, and downstream costs. Amongst the various delignification strategies, the enzymatic method has been demonstrated as a promising eco-friendly process with no inhibitor generation, although it is not highly efficient Aquisalibacillus elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0082
-
2,6-dimethoxyphenol pH 8.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0145
-
pyrogallol pH 8.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0161
-
syringaldazine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0161
-
2,5-xylidine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0251
-
Tannic acid pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0392
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0514
-
gallic acid pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0518
-
L-tyrosine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0686
-
hydroquinone pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.0773
-
catechol pH 9.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.102
-
L-Dopa pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 0.367
-
guaiacol pH 8.0, 40°C Aquisalibacillus elongatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
Aquisalibacillus elongatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 75000
-
nondenaturing PAGE Aquisalibacillus elongatus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 diethylene triamine amongst 42 solvents tested, significant deactivation is observed only in the presence of diethylene triamine (less than 50% of activity retains after 6 h incubation), which is probably due to the stripping-off of the crucial bound-water monolayer from the enzyme molecule essential for its activity Aquisalibacillus elongatus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Aquisalibacillus elongatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein carbohydrate content: 8.1% Aquisalibacillus elongatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 one-step purification Aquisalibacillus elongatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 498.9
-
pH 8.0, 40°C, substrate: 2,6-dimethoxyphenol Aquisalibacillus elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 2,5-xylidine + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 catechol + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 gallic acid + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 guaiacol + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 hydroquinone + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 L-DOPA + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 L-tyrosine + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 pyrogallol + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 syringaldazine + O2
-
Aquisalibacillus elongatus ?
-
?
1.10.3.2 tannic acid + O2
-
Aquisalibacillus elongatus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 75000, nondenaturing PAGE Aquisalibacillus elongatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 40
-
substrates: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine or 2,6-dimethoxyphenol Aquisalibacillus elongatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 25 45 stable Aquisalibacillus elongatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 56.3
-
guaiacol pH 8.0, 40°C Aquisalibacillus elongatus
1.10.3.2 284.4
-
2,5-xylidine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 318.8
-
L-Dopa pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 412.5
-
catechol pH 9.0, 40°C Aquisalibacillus elongatus
1.10.3.2 443.8
-
hydroquinone pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 462.5
-
L-tyrosine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 675
-
gallic acid pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 918.8
-
syringaldazine pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 1009.4
-
2,6-dimethoxyphenol pH 8.0, 40°C Aquisalibacillus elongatus
1.10.3.2 1231.3
-
pyrogallol pH 8.0, 40°C Aquisalibacillus elongatus
1.10.3.2 1471.9
-
Tannic acid pH 7.0, 40°C Aquisalibacillus elongatus
1.10.3.2 2150
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 40°C Aquisalibacillus elongatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Aquisalibacillus elongatus
1.10.3.2 7
-
substrate: syringaldazine Aquisalibacillus elongatus
1.10.3.2 8
-
substrate: 2,6-dimethoxyphenol Aquisalibacillus elongatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 5 10 stable at pH 5.0-10.0. Activity is drastically reduced at pH values lower than 5.0 after 6 h incubation Aquisalibacillus elongatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Aquisalibacillus elongatus isoelectric focusing
-
6.8