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Literature summary extracted from

  • Katane, M.; Kawata, T.; Nakayama, K.; Saitoh, Y.; Kaneko, Y.; Matsuda, S.; Saitoh, Y.; Miyamoto, T.; Sekine, M.; Homma, H.
    Characterization of the enzymatic and structural properties of human D-aspartate oxidase and comparison with those of the rat and mouse enzymes (2015), Biol. Pharm. Bull., 38, 298-305 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expressed in Escherichia coli BL21(DE3) pLysS cells Mus musculus
1.4.3.1 expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens
1.4.3.1 expressed in Escherichia coli BL21(DE3) pLysS cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.1 Aminooxyacetate 66.4% residual activity at 1 mM Homo sapiens
1.4.3.1 Aminooxyacetate
-
Mus musculus
1.4.3.1 Aminooxyacetate
-
Rattus norvegicus
1.4.3.1 Cu2+ 90.7% residual activity at 1 mM Homo sapiens
1.4.3.1 Fe2+ 86.5% residual activity at 1 mM Homo sapiens
1.4.3.1 L-Glu 89.3% residual activity at 1 mM Homo sapiens
1.4.3.1 malonate
-
Homo sapiens
1.4.3.1 malonate
-
Mus musculus
1.4.3.1 malonate
-
Rattus norvegicus
1.4.3.1 meso-tartrate
-
Homo sapiens
1.4.3.1 meso-tartrate
-
Mus musculus
1.4.3.1 meso-tartrate
-
Rattus norvegicus
1.4.3.1 additional information not inhibited by EDTA, ATP, ADP, AMP, cAMP, and L-Asp Homo sapiens
1.4.3.1 Ni2+ 93.3% residual activity at 1 mM Homo sapiens
1.4.3.1 pyridoxal 5'-phosphate 93.5% residual activity at 0.2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.1 2.1
-
D-Asp at pH 8.3 and 37°C Homo sapiens
1.4.3.1 2.26
-
D-Asp at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 7.37
-
D-Asp at pH 8.3 and 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.1 additional information not influenced by Na+, K+, Mg2+, Ca2+, Co2+, Mn2+, and Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.1 D-Asp + H2O + O2 Mus musculus high activity oxaloacetate + NH3 + H2O2
-
?
1.4.3.1 D-Asp + H2O + O2 Homo sapiens high activity oxaloacetate + NH3 + H2O2
-
?
1.4.3.1 D-Asp + H2O + O2 Rattus norvegicus high activity oxaloacetate + NH3 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Homo sapiens Q99489
-
-
1.4.3.1 Mus musculus Q0E9Q7
-
-
1.4.3.1 Rattus norvegicus D3ZDM7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.1 His GraviTrap column chromatography Mus musculus
1.4.3.1 His GraviTrap column chromatography Homo sapiens
1.4.3.1 His GraviTrap column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.1 NRK-52E cell
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.1 0.12
-
with D-Pro as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 0.14
-
with D-His as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 0.16
-
with D-Asn as substrate, at pH 8.3 and 37°C Mus musculus
1.4.3.1 0.46
-
with D-Glu as substrate, at pH 8.3 and 37°C Mus musculus
1.4.3.1 0.47
-
with N-methyl-L-Asp as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 2.93
-
with D-Asn as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 3.17
-
with N-methyl-D-Glu as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 7.82
-
with D-Glu as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 12.2
-
with D-Asp as substrate, at pH 8.3 and 37°C Mus musculus
1.4.3.1 13.1
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C Mus musculus
1.4.3.1 87.5
-
with D-Asp as substrate, at pH 8.3 and 37°C Homo sapiens
1.4.3.1 91.2
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 D-Asn + H2O + O2 low activity Mus musculus 2-oxosuccinamic acid + NH3 + H2O2
-
?
1.4.3.1 D-Asn + H2O + O2 low activity Homo sapiens 2-oxosuccinamic acid + NH3 + H2O2
-
?
1.4.3.1 D-Asp + H2O + O2 high activity Mus musculus oxaloacetate + NH3 + H2O2
-
?
1.4.3.1 D-Asp + H2O + O2 high activity Homo sapiens oxaloacetate + NH3 + H2O2
-
?
1.4.3.1 D-Asp + H2O + O2 high activity Rattus norvegicus oxaloacetate + NH3 + H2O2
-
?
1.4.3.1 D-Glu + H2O + O2 low activity Mus musculus 2-oxoglutarate + H2O2 + NH3
-
?
1.4.3.1 D-Glu + H2O + O2 low activity Homo sapiens 2-oxoglutarate + H2O2 + NH3
-
?
1.4.3.1 D-His + H2O + O2 low activity Mus musculus 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
?
1.4.3.1 D-His + H2O + O2 low activity Homo sapiens 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
?
1.4.3.1 D-Pro + H2O + O2 low activity Mus musculus 2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.1 D-Pro + H2O + O2 low activity Homo sapiens 2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.1 additional information the enzyme exhibits low activities towards D-Glu and N-methyl-D-Glu, D-Asn, D-Pro and D-His. There is no activity detected towards D-Gln, D-Ser, D-Thr, D-Tyr, D-Ala, D-Val, D-Leu, D-Ile, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, N-methyl-L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Tyr, L-Ala, L-Val, L-Leu, L-Ile, L-Pro, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, L-His, or Gly Homo sapiens ?
-
?
1.4.3.1 additional information the enzyme exhibits no activity towards D-Gln, D-Ser, D-Thr, D-Ala, D-Val, D-Leu, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Ala, L-Val, L-Leu, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, or L-His Mus musculus ?
-
?
1.4.3.1 N-methyl-D-Asp + H2O + O2 high activity Mus musculus oxaloacetate + methylamine + H2O2
-
?
1.4.3.1 N-methyl-D-Asp + H2O + O2 high activity Homo sapiens oxaloacetate + methylamine + H2O2
-
?
1.4.3.1 N-methyl-D-Glu + H2O + O2 low activity Mus musculus 2-oxoglutarate + methylamine + H2O2
-
?
1.4.3.1 N-methyl-D-Glu + H2O + O2 low activity Homo sapiens 2-oxoglutarate + methylamine + H2O2
-
?
1.4.3.1 N-methyl-L-Asp + H2O + O2 low activity Mus musculus oxaloacetate + methylamine + H2O2
-
?
1.4.3.1 N-methyl-L-Asp + H2O + O2 low activity Homo sapiens oxaloacetate + methylamine + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.1 D-Asp oxidase
-
Mus musculus
1.4.3.1 D-Asp oxidase
-
Homo sapiens
1.4.3.1 D-Asp oxidase
-
Rattus norvegicus
1.4.3.1 DASPO
-
Mus musculus
1.4.3.1 DASPO
-
Homo sapiens
1.4.3.1 DASPO
-
Rattus norvegicus
1.4.3.1 DDO
-
Mus musculus
1.4.3.1 DDO
-
Homo sapiens
1.4.3.1 DDO
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.1 45
-
-
Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.1 30 60 after 30 min of incubation, there is no loss of activity until the preincubation temperature reaches 45°C, and 9% of the maximum activity is lost at 50°C. The enzyme retains half of its maximum activity at 55°C, while almost all of the activity is lost at 60°C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.1 9.83
-
D-Asp at pH 8.3 and 37°C Mus musculus
1.4.3.1 31.1
-
D-Asp at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 68.4
-
D-Asp at pH 8.3 and 37°C Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.1 6 12.5 the activity increases gradually as the pH is increased from 6.0 to 8.3, remains approximately constant at pH 8.3 to 12.5, and decreases rapidly when the pH is greater than 12.5. No enzymatic activity is detected at very low and high pH levels (5.0 and 13.0), respectively Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.1 7.5 12 after 30 min incubation time, the enzyme is stable at pH 8.3 to 11.0 and retains 80% and 60% of its maximum activity at pH 7.5 and pH 12.0, respectively Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.1 FAD
-
Mus musculus
1.4.3.1 FAD
-
Homo sapiens
1.4.3.1 FAD
-
Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.1 0.153
-
malonate at pH 8.3 and 37°C Homo sapiens
1.4.3.1 0.472
-
meso-tartrate at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 0.681
-
meso-tartrate at pH 8.3 and 37°C Homo sapiens
1.4.3.1 1.22
-
malonate at pH 8.3 and 37°C Mus musculus
1.4.3.1 1.416
-
Aminooxyacetate at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 1.492
-
Aminooxyacetate at pH 8.3 and 37°C Homo sapiens
1.4.3.1 1.562
-
malonate at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 1.915
-
Aminooxyacetate at pH 8.3 and 37°C Mus musculus
1.4.3.1 2.089
-
meso-tartrate at pH 8.3 and 37°C Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.1 1.354
-
D-Asp at pH 8.3 and 37°C Mus musculus
1.4.3.1 14.104
-
D-Asp at pH 8.3 and 37°C Rattus norvegicus
1.4.3.1 32.651
-
D-Asp at pH 8.3 and 37°C Homo sapiens