BRENDA - Enzyme Database

Characterization of the enzymatic and structural properties of human D-aspartate oxidase and comparison with those of the rat and mouse enzymes

Katane, M.; Kawata, T.; Nakayama, K.; Saitoh, Y.; Kaneko, Y.; Matsuda, S.; Saitoh, Y.; Miyamoto, T.; Sekine, M.; Homma, H.; Biol. Pharm. Bull. 38, 298-305 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Homo sapiens
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Mus musculus
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
Aminooxyacetate
66.4% residual activity at 1 mM
Homo sapiens
1.4.3.1
Aminooxyacetate
-
Mus musculus
1.4.3.1
Aminooxyacetate
-
Rattus norvegicus
1.4.3.1
Cu2+
90.7% residual activity at 1 mM
Homo sapiens
1.4.3.1
Fe2+
86.5% residual activity at 1 mM
Homo sapiens
1.4.3.1
L-Glu
89.3% residual activity at 1 mM
Homo sapiens
1.4.3.1
malonate
-
Homo sapiens
1.4.3.1
malonate
-
Mus musculus
1.4.3.1
malonate
-
Rattus norvegicus
1.4.3.1
meso-tartrate
-
Homo sapiens
1.4.3.1
meso-tartrate
-
Mus musculus
1.4.3.1
meso-tartrate
-
Rattus norvegicus
1.4.3.1
additional information
not inhibited by EDTA, ATP, ADP, AMP, cAMP, and L-Asp
Homo sapiens
1.4.3.1
Ni2+
93.3% residual activity at 1 mM
Homo sapiens
1.4.3.1
pyridoxal 5'-phosphate
93.5% residual activity at 0.2 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
2.1
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
2.26
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
7.37
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.1
additional information
not influenced by Na+, K+, Mg2+, Ca2+, Co2+, Mn2+, and Zn2+
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-Asp + H2O + O2
Mus musculus
high activity
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-Asp + H2O + O2
Homo sapiens
high activity
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-Asp + H2O + O2
Rattus norvegicus
high activity
oxaloacetate + NH3 + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.1
Homo sapiens
Q99489
-
-
1.4.3.1
Mus musculus
Q0E9Q7
-
-
1.4.3.1
Rattus norvegicus
D3ZDM7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.1
His GraviTrap column chromatography
Homo sapiens
1.4.3.1
His GraviTrap column chromatography
Mus musculus
1.4.3.1
His GraviTrap column chromatography
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.1
NRK-52E cell
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
0.12
-
with D-Pro as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.14
-
with D-His as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.16
-
with D-Asn as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
0.46
-
with D-Glu as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
0.47
-
with N-methyl-L-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
2.93
-
with D-Asn as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
3.17
-
with N-methyl-D-Glu as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
7.82
-
with D-Glu as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
12.2
-
with D-Asp as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
13.1
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
87.5
-
with D-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
91.2
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-Asn + H2O + O2
low activity
742035
Mus musculus
2-oxosuccinamic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asn + H2O + O2
low activity
742035
Homo sapiens
2-oxosuccinamic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Mus musculus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Homo sapiens
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Rattus norvegicus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Glu + H2O + O2
low activity
742035
Mus musculus
2-oxoglutarate + H2O2 + NH3
-
-
-
?
1.4.3.1
D-Glu + H2O + O2
low activity
742035
Homo sapiens
2-oxoglutarate + H2O2 + NH3
-
-
-
?
1.4.3.1
D-His + H2O + O2
low activity
742035
Mus musculus
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-His + H2O + O2
low activity
742035
Homo sapiens
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Pro + H2O + O2
low activity
742035
Mus musculus
2-oxopentanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Pro + H2O + O2
low activity
742035
Homo sapiens
2-oxopentanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
additional information
the enzyme exhibits low activities towards D-Glu and N-methyl-D-Glu, D-Asn, D-Pro and D-His. There is no activity detected towards D-Gln, D-Ser, D-Thr, D-Tyr, D-Ala, D-Val, D-Leu, D-Ile, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, N-methyl-L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Tyr, L-Ala, L-Val, L-Leu, L-Ile, L-Pro, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, L-His, or Gly
742035
Homo sapiens
?
-
-
-
-
1.4.3.1
additional information
the enzyme exhibits no activity towards D-Gln, D-Ser, D-Thr, D-Ala, D-Val, D-Leu, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Ala, L-Val, L-Leu, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, or L-His
742035
Mus musculus
?
-
-
-
-
1.4.3.1
N-methyl-D-Asp + H2O + O2
high activity
742035
Mus musculus
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Asp + H2O + O2
high activity
742035
Homo sapiens
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Glu + H2O + O2
low activity
742035
Mus musculus
2-oxoglutarate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Glu + H2O + O2
low activity
742035
Homo sapiens
2-oxoglutarate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-L-Asp + + H2O + O2
low activity
742035
Mus musculus
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-L-Asp + + H2O + O2
low activity
742035
Homo sapiens
oxaloacetate + methylamine + H2O2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
45
-
-
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.1
30
60
after 30 min of incubation, there is no loss of activity until the preincubation temperature reaches 45°C, and 9% of the maximum activity is lost at 50°C. The enzyme retains half of its maximum activity at 55°C, while almost all of the activity is lost at 60°C
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.1
9.83
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
31.1
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
68.4
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.3.1
6
12.5
the activity increases gradually as the pH is increased from 6.0 to 8.3, remains approximately constant at pH 8.3 to 12.5, and decreases rapidly when the pH is greater than 12.5. No enzymatic activity is detected at very low and high pH levels (5.0 and 13.0), respectively
Homo sapiens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.1
7.5
12
after 30 min incubation time, the enzyme is stable at pH 8.3 to 11.0 and retains 80% and 60% of its maximum activity at pH 7.5 and pH 12.0, respectively
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.1
FAD
-
Homo sapiens
1.4.3.1
FAD
-
Rattus norvegicus
1.4.3.1
FAD
-
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
0.153
-
malonate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.472
-
meso-tartrate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
0.681
-
meso-tartrate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
1.22
-
malonate
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
1.416
-
Aminooxyacetate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
1.492
-
Aminooxyacetate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
1.562
-
malonate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
1.915
-
Aminooxyacetate
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
2.089
-
meso-tartrate
at pH 8.3 and 37°C
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Homo sapiens
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Mus musculus
1.4.3.1
expressed in Escherichia coli BL21(DE3) pLysS cells
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.1
FAD
-
Homo sapiens
1.4.3.1
FAD
-
Rattus norvegicus
1.4.3.1
FAD
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
Aminooxyacetate
66.4% residual activity at 1 mM
Homo sapiens
1.4.3.1
Aminooxyacetate
-
Mus musculus
1.4.3.1
Aminooxyacetate
-
Rattus norvegicus
1.4.3.1
Cu2+
90.7% residual activity at 1 mM
Homo sapiens
1.4.3.1
Fe2+
86.5% residual activity at 1 mM
Homo sapiens
1.4.3.1
L-Glu
89.3% residual activity at 1 mM
Homo sapiens
1.4.3.1
malonate
-
Homo sapiens
1.4.3.1
malonate
-
Mus musculus
1.4.3.1
malonate
-
Rattus norvegicus
1.4.3.1
meso-tartrate
-
Homo sapiens
1.4.3.1
meso-tartrate
-
Mus musculus
1.4.3.1
meso-tartrate
-
Rattus norvegicus
1.4.3.1
additional information
not inhibited by EDTA, ATP, ADP, AMP, cAMP, and L-Asp
Homo sapiens
1.4.3.1
Ni2+
93.3% residual activity at 1 mM
Homo sapiens
1.4.3.1
pyridoxal 5'-phosphate
93.5% residual activity at 0.2 mM
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
0.153
-
malonate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.472
-
meso-tartrate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
0.681
-
meso-tartrate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
1.22
-
malonate
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
1.416
-
Aminooxyacetate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
1.492
-
Aminooxyacetate
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
1.562
-
malonate
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
1.915
-
Aminooxyacetate
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
2.089
-
meso-tartrate
at pH 8.3 and 37°C
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
2.1
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
2.26
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
7.37
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.1
additional information
not influenced by Na+, K+, Mg2+, Ca2+, Co2+, Mn2+, and Zn2+
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-Asp + H2O + O2
Mus musculus
high activity
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-Asp + H2O + O2
Homo sapiens
high activity
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-Asp + H2O + O2
Rattus norvegicus
high activity
oxaloacetate + NH3 + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
His GraviTrap column chromatography
Homo sapiens
1.4.3.1
His GraviTrap column chromatography
Mus musculus
1.4.3.1
His GraviTrap column chromatography
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.1
NRK-52E cell
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
0.12
-
with D-Pro as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.14
-
with D-His as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
0.16
-
with D-Asn as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
0.46
-
with D-Glu as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
0.47
-
with N-methyl-L-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
2.93
-
with D-Asn as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
3.17
-
with N-methyl-D-Glu as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
7.82
-
with D-Glu as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
12.2
-
with D-Asp as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
13.1
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C
Mus musculus
1.4.3.1
87.5
-
with D-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
1.4.3.1
91.2
-
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-Asn + H2O + O2
low activity
742035
Mus musculus
2-oxosuccinamic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asn + H2O + O2
low activity
742035
Homo sapiens
2-oxosuccinamic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Mus musculus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Homo sapiens
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Asp + H2O + O2
high activity
742035
Rattus norvegicus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Glu + H2O + O2
low activity
742035
Mus musculus
2-oxoglutarate + H2O2 + NH3
-
-
-
?
1.4.3.1
D-Glu + H2O + O2
low activity
742035
Homo sapiens
2-oxoglutarate + H2O2 + NH3
-
-
-
?
1.4.3.1
D-His + H2O + O2
low activity
742035
Mus musculus
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-His + H2O + O2
low activity
742035
Homo sapiens
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Pro + H2O + O2
low activity
742035
Mus musculus
2-oxopentanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
D-Pro + H2O + O2
low activity
742035
Homo sapiens
2-oxopentanoic acid + NH3 + H2O2
-
-
-
?
1.4.3.1
additional information
the enzyme exhibits low activities towards D-Glu and N-methyl-D-Glu, D-Asn, D-Pro and D-His. There is no activity detected towards D-Gln, D-Ser, D-Thr, D-Tyr, D-Ala, D-Val, D-Leu, D-Ile, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, N-methyl-L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Tyr, L-Ala, L-Val, L-Leu, L-Ile, L-Pro, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, L-His, or Gly
742035
Homo sapiens
?
-
-
-
-
1.4.3.1
additional information
the enzyme exhibits no activity towards D-Gln, D-Ser, D-Thr, D-Ala, D-Val, D-Leu, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Ala, L-Val, L-Leu, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, or L-His
742035
Mus musculus
?
-
-
-
-
1.4.3.1
N-methyl-D-Asp + H2O + O2
high activity
742035
Mus musculus
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Asp + H2O + O2
high activity
742035
Homo sapiens
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Glu + H2O + O2
low activity
742035
Mus musculus
2-oxoglutarate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-Glu + H2O + O2
low activity
742035
Homo sapiens
2-oxoglutarate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-L-Asp + + H2O + O2
low activity
742035
Mus musculus
oxaloacetate + methylamine + H2O2
-
-
-
?
1.4.3.1
N-methyl-L-Asp + + H2O + O2
low activity
742035
Homo sapiens
oxaloacetate + methylamine + H2O2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
45
-
-
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.1
30
60
after 30 min of incubation, there is no loss of activity until the preincubation temperature reaches 45°C, and 9% of the maximum activity is lost at 50°C. The enzyme retains half of its maximum activity at 55°C, while almost all of the activity is lost at 60°C
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.1
9.83
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
31.1
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
68.4
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.3.1
6
12.5
the activity increases gradually as the pH is increased from 6.0 to 8.3, remains approximately constant at pH 8.3 to 12.5, and decreases rapidly when the pH is greater than 12.5. No enzymatic activity is detected at very low and high pH levels (5.0 and 13.0), respectively
Homo sapiens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.1
7.5
12
after 30 min incubation time, the enzyme is stable at pH 8.3 to 11.0 and retains 80% and 60% of its maximum activity at pH 7.5 and pH 12.0, respectively
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4.3.1
1.354
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
14.104
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
32.651
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4.3.1
1.354
-
D-Asp
at pH 8.3 and 37°C
Mus musculus
1.4.3.1
14.104
-
D-Asp
at pH 8.3 and 37°C
Rattus norvegicus
1.4.3.1
32.651
-
D-Asp
at pH 8.3 and 37°C
Homo sapiens