EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.1 | expressed in Escherichia coli BL21(DE3) pLysS cells | Mus musculus |
1.4.3.1 | expressed in Escherichia coli BL21(DE3) pLysS cells | Homo sapiens |
1.4.3.1 | expressed in Escherichia coli BL21(DE3) pLysS cells | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.1 | Aminooxyacetate | 66.4% residual activity at 1 mM | Homo sapiens | |
1.4.3.1 | Aminooxyacetate | - |
Mus musculus | |
1.4.3.1 | Aminooxyacetate | - |
Rattus norvegicus | |
1.4.3.1 | Cu2+ | 90.7% residual activity at 1 mM | Homo sapiens | |
1.4.3.1 | Fe2+ | 86.5% residual activity at 1 mM | Homo sapiens | |
1.4.3.1 | L-Glu | 89.3% residual activity at 1 mM | Homo sapiens | |
1.4.3.1 | malonate | - |
Homo sapiens | |
1.4.3.1 | malonate | - |
Mus musculus | |
1.4.3.1 | malonate | - |
Rattus norvegicus | |
1.4.3.1 | meso-tartrate | - |
Homo sapiens | |
1.4.3.1 | meso-tartrate | - |
Mus musculus | |
1.4.3.1 | meso-tartrate | - |
Rattus norvegicus | |
1.4.3.1 | additional information | not inhibited by EDTA, ATP, ADP, AMP, cAMP, and L-Asp | Homo sapiens | |
1.4.3.1 | Ni2+ | 93.3% residual activity at 1 mM | Homo sapiens | |
1.4.3.1 | pyridoxal 5'-phosphate | 93.5% residual activity at 0.2 mM | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.1 | 2.1 | - |
D-Asp | at pH 8.3 and 37°C | Homo sapiens | |
1.4.3.1 | 2.26 | - |
D-Asp | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 7.37 | - |
D-Asp | at pH 8.3 and 37°C | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.1 | additional information | not influenced by Na+, K+, Mg2+, Ca2+, Co2+, Mn2+, and Zn2+ | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.1 | D-Asp + H2O + O2 | Mus musculus | high activity | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asp + H2O + O2 | Homo sapiens | high activity | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asp + H2O + O2 | Rattus norvegicus | high activity | oxaloacetate + NH3 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.1 | Homo sapiens | Q99489 | - |
- |
1.4.3.1 | Mus musculus | Q0E9Q7 | - |
- |
1.4.3.1 | Rattus norvegicus | D3ZDM7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.1 | His GraviTrap column chromatography | Mus musculus |
1.4.3.1 | His GraviTrap column chromatography | Homo sapiens |
1.4.3.1 | His GraviTrap column chromatography | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.1 | NRK-52E cell | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.3.1 | 0.12 | - |
with D-Pro as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 0.14 | - |
with D-His as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 0.16 | - |
with D-Asn as substrate, at pH 8.3 and 37°C | Mus musculus |
1.4.3.1 | 0.46 | - |
with D-Glu as substrate, at pH 8.3 and 37°C | Mus musculus |
1.4.3.1 | 0.47 | - |
with N-methyl-L-Asp as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 2.93 | - |
with D-Asn as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 3.17 | - |
with N-methyl-D-Glu as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 7.82 | - |
with D-Glu as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 12.2 | - |
with D-Asp as substrate, at pH 8.3 and 37°C | Mus musculus |
1.4.3.1 | 13.1 | - |
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C | Mus musculus |
1.4.3.1 | 87.5 | - |
with D-Asp as substrate, at pH 8.3 and 37°C | Homo sapiens |
1.4.3.1 | 91.2 | - |
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.1 | D-Asn + H2O + O2 | low activity | Mus musculus | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asn + H2O + O2 | low activity | Homo sapiens | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asp + H2O + O2 | high activity | Mus musculus | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asp + H2O + O2 | high activity | Homo sapiens | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Asp + H2O + O2 | high activity | Rattus norvegicus | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Glu + H2O + O2 | low activity | Mus musculus | 2-oxoglutarate + H2O2 + NH3 | - |
? | |
1.4.3.1 | D-Glu + H2O + O2 | low activity | Homo sapiens | 2-oxoglutarate + H2O2 + NH3 | - |
? | |
1.4.3.1 | D-His + H2O + O2 | low activity | Mus musculus | 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-His + H2O + O2 | low activity | Homo sapiens | 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Pro + H2O + O2 | low activity | Mus musculus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-Pro + H2O + O2 | low activity | Homo sapiens | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | additional information | the enzyme exhibits low activities towards D-Glu and N-methyl-D-Glu, D-Asn, D-Pro and D-His. There is no activity detected towards D-Gln, D-Ser, D-Thr, D-Tyr, D-Ala, D-Val, D-Leu, D-Ile, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, N-methyl-L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Tyr, L-Ala, L-Val, L-Leu, L-Ile, L-Pro, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, L-His, or Gly | Homo sapiens | ? | - |
? | |
1.4.3.1 | additional information | the enzyme exhibits no activity towards D-Gln, D-Ser, D-Thr, D-Ala, D-Val, D-Leu, D-Phe, D-Met, D-Trp, D-cysteine, D-Lys, D-Arg, L-Asp, L-Glu, L-Asn, L-Gln, L-Ser, L-Thr, L-Ala, L-Val, L-Leu, L-Phe, L-Met, L-Trp, L-cysteine, L-Lys, L-Arg, or L-His | Mus musculus | ? | - |
? | |
1.4.3.1 | N-methyl-D-Asp + H2O + O2 | high activity | Mus musculus | oxaloacetate + methylamine + H2O2 | - |
? | |
1.4.3.1 | N-methyl-D-Asp + H2O + O2 | high activity | Homo sapiens | oxaloacetate + methylamine + H2O2 | - |
? | |
1.4.3.1 | N-methyl-D-Glu + H2O + O2 | low activity | Mus musculus | 2-oxoglutarate + methylamine + H2O2 | - |
? | |
1.4.3.1 | N-methyl-D-Glu + H2O + O2 | low activity | Homo sapiens | 2-oxoglutarate + methylamine + H2O2 | - |
? | |
1.4.3.1 | N-methyl-L-Asp + H2O + O2 | low activity | Mus musculus | oxaloacetate + methylamine + H2O2 | - |
? | |
1.4.3.1 | N-methyl-L-Asp + H2O + O2 | low activity | Homo sapiens | oxaloacetate + methylamine + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.1 | D-Asp oxidase | - |
Mus musculus |
1.4.3.1 | D-Asp oxidase | - |
Homo sapiens |
1.4.3.1 | D-Asp oxidase | - |
Rattus norvegicus |
1.4.3.1 | DASPO | - |
Mus musculus |
1.4.3.1 | DASPO | - |
Homo sapiens |
1.4.3.1 | DASPO | - |
Rattus norvegicus |
1.4.3.1 | DDO | - |
Mus musculus |
1.4.3.1 | DDO | - |
Homo sapiens |
1.4.3.1 | DDO | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.1 | 45 | - |
- |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.1 | 30 | 60 | after 30 min of incubation, there is no loss of activity until the preincubation temperature reaches 45°C, and 9% of the maximum activity is lost at 50°C. The enzyme retains half of its maximum activity at 55°C, while almost all of the activity is lost at 60°C | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.1 | 9.83 | - |
D-Asp | at pH 8.3 and 37°C | Mus musculus | |
1.4.3.1 | 31.1 | - |
D-Asp | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 68.4 | - |
D-Asp | at pH 8.3 and 37°C | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.1 | 6 | 12.5 | the activity increases gradually as the pH is increased from 6.0 to 8.3, remains approximately constant at pH 8.3 to 12.5, and decreases rapidly when the pH is greater than 12.5. No enzymatic activity is detected at very low and high pH levels (5.0 and 13.0), respectively | Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.1 | 7.5 | 12 | after 30 min incubation time, the enzyme is stable at pH 8.3 to 11.0 and retains 80% and 60% of its maximum activity at pH 7.5 and pH 12.0, respectively | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.1 | FAD | - |
Mus musculus | |
1.4.3.1 | FAD | - |
Homo sapiens | |
1.4.3.1 | FAD | - |
Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.1 | 0.153 | - |
malonate | at pH 8.3 and 37°C | Homo sapiens | |
1.4.3.1 | 0.472 | - |
meso-tartrate | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 0.681 | - |
meso-tartrate | at pH 8.3 and 37°C | Homo sapiens | |
1.4.3.1 | 1.22 | - |
malonate | at pH 8.3 and 37°C | Mus musculus | |
1.4.3.1 | 1.416 | - |
Aminooxyacetate | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 1.492 | - |
Aminooxyacetate | at pH 8.3 and 37°C | Homo sapiens | |
1.4.3.1 | 1.562 | - |
malonate | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 1.915 | - |
Aminooxyacetate | at pH 8.3 and 37°C | Mus musculus | |
1.4.3.1 | 2.089 | - |
meso-tartrate | at pH 8.3 and 37°C | Mus musculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.1 | 1.354 | - |
D-Asp | at pH 8.3 and 37°C | Mus musculus | |
1.4.3.1 | 14.104 | - |
D-Asp | at pH 8.3 and 37°C | Rattus norvegicus | |
1.4.3.1 | 32.651 | - |
D-Asp | at pH 8.3 and 37°C | Homo sapiens |