EC Number | Cloned (Comment) | Organism |
---|---|---|
7.1.1.2 | expression in Thermus thermophilus and in Eschrichia coli | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.2 | additional information | not inhibitory: rotenone; not inhibitory: rotenone | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.1.1.2 | 0.017 | - |
menadione | pH 7.5, 60°C | Thermus thermophilus | |
7.1.1.2 | 0.024 | - |
NADH | pH 7.5, 60°C | Thermus thermophilus | |
7.1.1.2 | 0.032 | - |
menadione | pH 7.5, 60°C | Thermus thermophilus | |
7.1.1.2 | 0.05 | - |
NADH | pH 7.5, 60°C | Thermus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.1.1.2 | membrane | - |
Thermus thermophilus | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.1.2 | Thermus thermophilus | Q70DV6 | - |
- |
7.1.1.2 | Thermus thermophilus | Q72HK3 | - |
- |
7.1.1.2 | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q70DV6 | - |
- |
7.1.1.2 | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72HK3 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.1.1.2 | 1.17 | - |
60°C, pH 7.5, recombinant protein expressed in Escherichia coli, presence of FAD | Thermus thermophilus |
7.1.1.2 | 1.23 | - |
60°C, pH 7.5, recombinant protein expressed in Escherichia coli, presence of FAD | Thermus thermophilus |
7.1.1.2 | 20 | - |
60°C, pH 7.5, recombinant protein expressed in Thermus thermopilus, presence of FAD | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.1.2 | NADH + H+ + decylubiquinone | - |
Thermus thermophilus | NAD+ + decylubiquinol | - |
? | |
7.1.1.2 | NADH + H+ + decylubiquinone | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NAD+ + decylubiquinol | - |
? | |
7.1.1.2 | NADH + H+ + duroquinone | - |
Thermus thermophilus | NAD+ + duroquinol | - |
? | |
7.1.1.2 | NADH + H+ + duroquinone | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NAD+ + duroquinol | - |
? | |
7.1.1.2 | NADH + H+ + menadione | - |
Thermus thermophilus | NAD+ + menadiol | - |
? | |
7.1.1.2 | NADH + H+ + menadione | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NAD+ + menadiol | - |
? | |
7.1.1.2 | NADH + H+ + ubiquinone-1 | - |
Thermus thermophilus | NAD+ + ubiquinol-1 | - |
? | |
7.1.1.2 | NADH + H+ + ubiquinone-1 | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NAD+ + ubiquinol-1 | - |
? | |
7.1.1.2 | NADH + H+ + vitamin K2 | - |
Thermus thermophilus | NAD+ + reduced vitamin K2 | - |
? | |
7.1.1.2 | NADH + H+ + vitamin K2 | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NAD+ + reduced vitamin K2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.1.1.2 | ? | x * 43000, calculated | Thermus thermophilus |
7.1.1.2 | ? | x * 48000, SDS-PAGE and calculated | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.1.2 | NDH-2A | - |
Thermus thermophilus |
7.1.1.2 | NrcN | - |
Thermus thermophilus |
7.1.1.2 | TT_C1484 | - |
Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.1.1.2 | 0.9 | - |
NADH | pH 7.5, 60°C | Thermus thermophilus | |
7.1.1.2 | 1.1 | - |
NADH | pH 7.5, 60°C | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.2 | FAD | FAD content of homologously produced NrcN is 1 mol of FAD/mol protein. FAD is not covalently bound | Thermus thermophilus | |
7.1.1.2 | FAD | UV-Vis spectrum shows a shoulder at 465 nm. FAD is not covalently bound | Thermus thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.1.1.2 | physiological function | protein NrcN functions independently of other components of the putative Nrc complex | Thermus thermophilus |