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Literature summary extracted from

  • Volkov, P.V.; Rozhkova, A.M.; Gusakov, A.V.; Zorov, I.N.; Sinitsyn, A.P.
    Glucoamylases from Penicillium verruculosum and Myceliophthora thermophila analysis of differences in activity against polymeric substrates based on 3D model structures of the intact enzymes (2015), Biochimie, 110, 45-51 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Penicillium canescens Talaromyces verruculosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.3 modeling of structure based on PDB entry 2vn7 Talaromyces verruculosus
3.2.1.3 modeling of structure based on PDB entry 2vn7 Thermothelomyces thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Talaromyces verruculosus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Talaromyces verruculosus A0A1L5IUR2
-
-
3.2.1.3 Thermothelomyces thermophilus G2QPS0
-
-
3.2.1.3 Thermothelomyces thermophilus DSM 1799 G2QPS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 0.3
-
 substrate maltose, pH 4.7, 40°C Talaromyces verruculosus
3.2.1.3 3.6
-
 substrate amylose, pH 4.7, 58°C Talaromyces verruculosus
3.2.1.3 4.7
-
 substrate starch, pH 4.7, 30°C Talaromyces verruculosus
3.2.1.3 4.8
-
 substrate amylopectin, pH 4.7, 58°C Talaromyces verruculosus
3.2.1.3 5.2
-
 substrate maltose, pH 4.7, 40°C Thermothelomyces thermophilus
3.2.1.3 7.8
-
 substrate amylose, pH 4.7, 58°C Thermothelomyces thermophilus
3.2.1.3 9.5
-
 substrate amylopectin, pH 4.7, 58°C Thermothelomyces thermophilus
3.2.1.3 25
-
 substrate starch, pH 4.7, 30°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Thermothelomyces thermophilus DSM 1799 ?
-
 ?
3.2.1.3 amylose + H2O
-
 Talaromyces verruculosus D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Thermothelomyces thermophilus D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Thermothelomyces thermophilus DSM 1799 D-glucose + ?
-
 ?
3.2.1.3 maltose + H2O
-
 Talaromyces verruculosus 2 D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Thermothelomyces thermophilus 2 D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Thermothelomyces thermophilus DSM 1799 2 D-glucose
-
 ?
3.2.1.3 additional information no substrate: 4-nitrophenyl alpha-D-glucopyranoside Talaromyces verruculosus ?
-
 ?
3.2.1.3 additional information no substrate: 4-nitrophenyl alpha-D-glucopyranoside Thermothelomyces thermophilus ?
-
 ?
3.2.1.3 additional information no substrate: 4-nitrophenyl alpha-D-glucopyranoside Thermothelomyces thermophilus DSM 1799 ?
-
 ?
3.2.1.3 starch + H2O
-
 Talaromyces verruculosus D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Thermothelomyces thermophilus D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Thermothelomyces thermophilus DSM 1799 D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 88000, SDS-PAGE, x * 65989, calculated Talaromyces verruculosus
3.2.1.3 ? x * 88000, SDS-PAGE, x * 65989, calculated Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 Gla1
-
 Talaromyces verruculosus
3.2.1.3 Gla1
-
 Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4
-
-
 Talaromyces verruculosus
3.2.1.3 5.5
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 4.7
-
 90% of maximum activity Talaromyces verruculosus
3.2.1.3 4.7
-
 90% of maximum activity Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Talaromyces verruculosus isoelectric focusing
-
 3.8
3.2.1.3 Thermothelomyces thermophilus calculated
-
 3.8
3.2.1.3 Talaromyces verruculosus calculated
-
 4.6
3.2.1.3 Thermothelomyces thermophilus isoelectric focusing
-
 5.1