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Literature summary extracted from

  • Li, M.; Khan, S.; Rong, H.; Tuma, R.; Hatzakis, N.S.; Jeuken, L.J.C.
    Effects of membrane curvature and pH on proton pumping activity of single cytochrome bo3 enzymes (2017), Biochim. Biophys. Acta, 1858, 763-770 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.3 expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.3 E286C the mutant still reduces oxygen to oxidize ubiquinol, transporting chemical protons across the membrane in the process, but is unable to pump protons Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.3 membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.3 ubiquinol + O2 + H+[side 1] Escherichia coli
-
 ubiquinone + H2O + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.3 Ni-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 ubiquinol + O2 + H+[side 1]
-
 Escherichia coli ubiquinone + H2O + H+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 cytochrome bo3
-
 Escherichia coli