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Literature summary extracted from

  • Lucio-Eterovic, A.K.; Jorge, J.A.; Polizeli, M.d.e. .L.; Terenzi, H.F.
    Biochemical characterisation of the trehalase of thermophilic fungi an enzyme with mixed properties of neutral and acid trehalase (2005), Biochim. Biophys. Acta, 1723, 201-207 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.28 Ca2+ at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Mycothermus thermophilus
3.2.1.28 Ca2+ at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Trichocladium griseum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ATP at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Mycothermus thermophilus
3.2.1.28 ATP at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Trichocladium griseum
3.2.1.28 EDTA at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Mycothermus thermophilus
3.2.1.28 EDTA at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP Trichocladium griseum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 extracellular
-
Mycothermus thermophilus
-
-
3.2.1.28 intracellular
-
Mycothermus thermophilus 5622
-
3.2.1.28 intracellular
-
Trichocladium griseum 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Mycothermus thermophilus
-
-
-
3.2.1.28 Trichocladium griseum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 3.2
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 4.8
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 5.2
-
40°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 7.7
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 7.7
-
40°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 10.8
-
40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 11.2
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 11.5
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 12.3
-
40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 12.4
-
60°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 16.5
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 18.4
-
40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 19.5
-
40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 25.6
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 26.2
-
60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 27
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 31.5
-
40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 31.5
-
60°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 42.2
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 46
-
40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 48.6
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 49.6
-
60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 76.5
-
60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 76.8
-
60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 95.5
-
40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 100
-
40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 113.4
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition Mycothermus thermophilus
3.2.1.28 154
-
60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition Mycothermus thermophilus
3.2.1.28 184
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum
3.2.1.28 195.5
-
40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 274.8
-
60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition Trichocladium griseum
3.2.1.28 389
-
40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition Trichocladium griseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
Mycothermus thermophilus 2 D-glucose
-
?
3.2.1.28 alpha,alpha-trehalose + H2O
-
Trichocladium griseum 2 D-glucose
-
?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 4.5
-
at pH 4.5, enzyme is neither activated by calcium nor inhibited by EDTA or ATP. Mycothermus thermophilus
3.2.1.28 4.5
-
at pH 4.5, enzyme is neither activated by calcium nor inhibited by EDTA or ATP. Trichocladium griseum
3.2.1.28 6.5
-
at p-h 6.5, enzyme is activated by calcium and inhibited by ATP Mycothermus thermophilus
3.2.1.28 6.5
-
at p-h 6.5, enzyme is activated by calcium and inhibited by ATP Trichocladium griseum